HAART对人类免疫缺陷病毒感染者淋巴细胞亚群的影响

Effect of highly active antiretroviral therapy on the lymphocyte subsets from peripheral blood of AIDS patients

应用流式细胞分析法通过分析检测41例AIDS患者与30例健康对照之间,以及21例AIDS患者在接受HAART治疗前后外周血淋巴细胞亚群的表达情况,研究感染HIV病毒以及HAART治疗对淋巴细胞亚群的影响。结果显示AIDS患者与健康对照相比,外周血CD4^+CD45RA^+、CD4^+CD28^+、CD4^+CD45RO^+、CD4^+CD95^+细胞比例明显低于正常对照,CD8^+CD95^+、CD8^+CD38^+、CD56^+细胞比例明显高于正常对照;经HAART治疗后,CD4^+CD45RA^+,CD4^+CD28^+细胞比例比治疗前升高,CD56^+细胞比例比治疗前显著下降。结果表明CD4^+T淋巴细胞表面CD45RA、CD28表达,以及CD56^+细胞能够预测AIDS患者HAART治疗效果。

To observe the changes of the peripheral blood lymphocyte subsets in AIDS patients receiving highly active antiretroviral therapy（HAART）, the expression of CD4^＋CD45RA^＋, CD4^＋ CD45RO^＋ , CD4^＋CD25^＋ , CD8^＋ CD25^＋, CD4^＋CD95^＋ , CD8^＋ CD95^＋ , CD4^＋ CD28^＋ , CD8^＋ CD38^＋ , CD14^＋, CD19^＋ , CD56^＋ lymphocyte subsets in peripheral blood were investigated by immunofluorescence staining method. In the AIDS patient, it was found that the percentages of CD4^＋ CD45RA^＋ , CD4^＋ CD28^＋ , CD4^＋ CD45RO^＋ , CD4^＋ CD95^＋ lymphocytes were significantly lower and that of CD56^＋ , CD8^＋ CD95^＋ , CD8^＋ CD38^＋ lymphocytes were significantly higher than that of healthy individuals. After treatment with HAART the percentages of CD4^＋ CD45RA^＋ and CD4^＋CD28^＋ lymphocyte elavated, and that of CD56^＋ lymphocytes decreased compared with that of the patients before HAART. These results suggest that the expression of CD45RA and CD28 on CD4^＋ T lymphocyte, and the CD56^＋ lymphocytes can predict the effect of HAART on AIDS patients.