摘要
目的探讨医院感染的耐甲氧西林金黄色葡萄球菌(MRSA)耐药基因的存在情况。方法对2006年6至7月临床分离的20株医院感染MRSA进行m ecA、tetM、aac(6′)/aph(2″)、aph(3′)Ⅲ、ant(4′4″)耐药基因检测。结果20株MRSA检出m ecA阳性率为100%,aac(6)′/aph(2″)阳性率75%,tetM阳性率70%,aph(3′)Ⅲ阳性率40%、ant(4′4″)阳性率为20%。结论多数MRSA菌株存在耐β-内酰胺类、四环素类、氨基糖苷类等多种抗生素耐药基因,与表型一致,表型与遗传学均支持MRSA具有耐多药特征。
Objective To investigate the existence of methicillin resistant Staphylococcus aureus(MRSA) drug-resistant gene inducing the nosocomial infection. Methods The drug-resistant gene including mecA, tetM, aac (6')/aph (2″), aph(3')Ⅲ, ant (4'4″) were detected in 20 strains of M RSA isolated from clinical infectious specimen between June and July in 2006. Results Among 20 stains of MRSA,the positive rates of mecA,tetM,aac(6')/aph(2″) ,aph (3') Ⅲ and ant(4'4″) were 100% ,70% ,75% ,40% and 20% respectively. Conclusions The genes resisting β-lactamase,tetracycline, aminoglycoside and other multi-antibiotic-resistant genes have already exited in the majority of strains ,which accords with the phenotypes. The phenotypes and the genetics support MRSA's characteristic of multidrug resistance.
出处
《检验医学》
CAS
北大核心
2007年第4期390-393,共4页
Laboratory Medicine
关键词
医院感染
耐甲氧西林
金黄色葡萄球菌
抗生素
耐药基因
Nosocomial infection
Methicillin resistant
Staphylococcus aureus
Antibiotic
Drug-resistant gene