依普拉芬对新生大鼠颅盖骨成骨细胞增殖和分化的影响

Effect of ipriflavone on the proliferation and differentiation of cultured rat osteoblast in vitro

目的研究依普拉芬对体外培养的原代大鼠成骨细胞增殖和分化功能的影响。方法体外分离培养新生大鼠颅盖骨成骨细胞,将不同浓度的依普拉芬加入培养液,测定细胞增殖情况、碱性磷酸酶(ALP)活性及钙化结节。结果与阴性对照组相比,各浓度组的依普拉芬均可增加成骨细胞数量(P<0.01),提高细胞的ALP活性和促进钙化结节形成(P<0.01),其中10-8～10-5mol/L作用更为显著。结论依普拉芬具有促进体外培养成骨细胞增殖、分化的作用。

Objective To study the effect of ipriflavone（7- isopropoxyisoflavone, IP） on the proliferation and differenti- ation of cultured rat osteoblast in vitro. Methods New born rat calvarid osteoblastic cell were isolated and cultured with the medium containing ipriflavone of different concentration. The proliferation, the activity of alkaline phosphatase（ALP） and calcium node were determined and compared with control. Results Compared with control, ipriflavone could increase the quantity of osteoblast（P〈0.01）. Ipriflavone could elevate the activity of ALP and ability of calcification（P〈0.01）. 10^-8～10^-5 mol/L ipriflavone had more significant effect than other concentration. Conclusion Ipriflavone could promote the proliferation and differentiation of cultured rat osteoblast in vitro.