黄山药愈伤组织的诱导与分化

Callus Induction and Differentiation of Dioscorea panthaica

研究黄山药愈伤组织形成、器官分化和植株再生,结果表明:叶片是诱导黄山药愈伤组织的最佳外植体;含2,4-D2.0 mg/L和BA2.0 mg/L的培养基对愈伤组织诱导效果最好;大多数愈伤组织在含有1.0 mg/LBA、0.5 mg/L NAA和2%蔗糖的培养基(pH值6.4)中能诱导出芽;含有0.5 mg/LBA、0.1 mg/L NAA和3%蔗糖的MS培养基(pH值6.8)能诱导大多数转接芽中根的形成。

The callus formation, organ differentiation and plantlet regeneration of Dioscorea panthaica were studied. The results showed that leaves were the best explants for callus induction; medium with 2.0 mg/L 2,4-D and 2.0 mg/L BA was optimal for callus induction; most callus showed shoot differentiation on medium with 1.0 mg/L BA, 0.5 mg/L NAA, 2% sucrose and pH 6.4, and medium with 0.5 mg/L BA, 0.1 mg/L NAA, 3% MS sucrose and pH 6.8 allowed for root formation in most of the sub cultured shoots.