兔角膜缘和口腔粘膜上皮细胞羊膜种植及生物学特性研究

Planting and biological character of corneal and oral epithelial cells cultivated on amniotic membrane

目的:研究兔角膜缘和口腔粘膜上皮细胞在羊膜上种植的方法及生物学特征。方法:将兔角膜缘上皮细胞分别种植于去上皮羊膜和完整羊膜、口腔粘膜上皮细胞种植于去上皮羊膜进行体外培养,对获得的复合组织进行组织学、超微结构观察和免疫组化检测。结果:两种上皮细胞在去上皮羊膜基底膜面都能黏附生长、增殖、汇合成片并分层,细胞表达角蛋白3。结论:兔角膜缘和口腔粘膜上皮细胞种植于去上皮羊膜获得的复合物具有分层的类角膜上皮结构,为解决眼表重建供体来源不足的矛盾带来了新的希望。

Objective：To investigate the cultivation and boilogical char-acter of corneal epithelial cells （ECs） and oral ECs planted on amniot-ic membrane （AM） as a substrate. Methods：Rabbit limbus ECs were planted on denuded AM and intact AM respectively. Oral Ecs were p-lanted on denuded AM.The cultivated epithelial sheet was examined by HE staining pathological section and electronmicroscopy （EM）,also immunohistochemically labeled with AE5 monoconal antibody to identify the expression of cytokeratin 3 （CK3）. Results：Rabbit corneal ECsgrew slowly and were difficult to stick and confluence on intact AM,while both rabbit corneal and oral ECs were easy to grow and proli-ferate on denuded AM wheather using tissue pieces or single cells.T-he resulted tissure was composed of the basement membrane of AM and multiple layers of ECs showing the presence of CK3. Conclusions：The stratification of corneal and oral epithelium layers attaching to the basement membrane of AM are similar to engineering tissue mak-ing a fundament to ocular surface reconstruction.