吖啶黄-罗丹明6G之间荧光能量转移及其在测定钒中的应用

Study on fluorescence resonance energy transfer between acridine yellow and rhodamine 6G and its application in the determination of vanadium

在λex／λem=465／556nm，阴离子表面活性剂十二烷基硫酸钠（SLS）存在下，吖啶黄-罗丹明6G间能够发生有效的能量转移，使罗丹明6G的荧光强度大大增强；在弱酸性条件下，五价钒的加入使能量转移体系罗丹明6G的荧光强度降低，即发生荧光猝灭。利用吖啶黄-罗丹明6G能量转移荧光猝灭法测定痕量钒，提高了测定钒的灵敏度和选择性。钒在5．85～87．74ug／L范围内与罗丹明6G荧光猝灭程度呈良好的线性关系，测定11份空白溶液，计算方法检出限为3．73μg／L。该方法用于饮用水和工业废水中痕量钒的测定，相对标准偏差为1．97％～3．95％，回收率为90．2％～104．6％。

A new method for the determination of trace vanadium was developed by using energy transfer from acridine yellow（AY）to rhodamine 6G（R6G）. It was found that at λex/λem=465/556 nm and in presence of H2SO4 and lauryl sodium sulfate（SLS）, the effective energy transfer could occur between AY and R6G. The fluorescence intensity of R6G was quenched by the reaction between vanadium and AY in sulfuric acid medium. The linear range of determination was 5.85- 87.74 μg/L for vanadium. The detection limit of this method was 3.73 μg/L（n = 11）. The relative standard deviation was 1.97%--3.95%. The recoveries were 90.2%-104.6%. The method has been applied to the determination of vanadium in drinking water and polluted water in industry with satisfactory results.