抗胃癌生物活性肽对荷胃癌裸鼠酯酶同工酶代谢的影响

Effect of Anti-GCBAP on Esterase Isoenzymes Metabolism in Gastric Cancer-Bearing Nude Mice

非特异性酯酶同工酶(x-NAE isoenzyme)活性的异常升高及异常同工酶谱的表达与恶性肿瘤的发生密切相关.抗胃癌生物活性肽是从处理后胃癌细胞诱导的相关动物脾脏中提取出来的一种新型生物学应答调节剂(BRM,Biological respo- nse modifier).实验表明,这一活性物质对胃癌细胞有很强的特异性抑制作用,并能在体内产生多种生物学效应.本文在此研究基础上,进一步探讨了其抗癌效应过程中对x-NAE同工酶代谢的影响,结果表明:体内注射抗胃癌生物活性肽,不仅使荷瘤鼠皮下肿瘤生长显著减慢(P<0.01),同时使x-NAE同工酶谱表型发生明显变化,部分同工酶带型消失.提示:这一抗胃癌生物活性肽对荷瘤小鼠体内x-NAE同工酶蛋白表达及其基因表达产生了明显的生物调节作用.

Abnormal elevation and abnormal isoenzyme of non-specific esterase (χ-NAE) are intimately associated with the genesis and development of cancer.Anti-GCBAP is a new kind of biological response modifier(BRM) which is separated from gastric cancer cells immunized animal spleen. As determined by clonogenic assay. Anti-GCBAP displayed highly-potent, specific cytotoxicity regarding to target cancer cells. In the present study, we have further investigated the effect of Anti-GCBAP on the χ-NAE isoenzymes in gastric cancer-bearing nude mice. The results demonstrated that in vivo adminstration of Anti-GCBAP can not only inhibit tumor growth in tumor-bearing mice, compared with control(p<0.01), but also change χ- NAE isoenzymes. The parts of the isoenzyme bands disappeared. It suggested that Anti-GCBAP produced singnificantly biological modulate on χ-NAE isoenzymes, and it may be one mechanisms of anti-tumor effect.