量子点技术在生物成像中的应用进展

Application of quantum dots technology in biological imaging

目的:介绍量子点材料的特点及其在生物成像中的应用,并对其前景进行展望。资料来源:应用计算机检索PubMed1998-01/2007-04关于量子点生物成像文章。检索词"quantum dots,biologicalimaging,bioconjugates,fluorescence imaging"限定文章的语言种类为"English"。资料选择:对资料进行初审,纳入标准:①量子点的特点及表面修饰。②量子点在不同的生物组织细胞中的成像应用。排除标准:重复性研究。资料提炼:共收集到符合上述要求的文献31篇,关于量子点的特点及表面修饰的14篇,关于量子点在不同的生物组织细胞中的成像应用17篇。资料综合:量子点(quantum dots,QDs)是一种新型纳米荧光材料,现有技术能将量子点与生物分子结合在一起作为一种高亮度而稳定的荧光探针用于生物成像。作为一种新型的荧光纳米材料量子点,在多种类型的生物成像研究中较传统的有机荧光素和荧光蛋白而言具有更加优越的特性,使研究者能够以一种全新的方法对单个细胞、组织、甚至活动物的基因、蛋白质和药物靶点进行研究,为疾病机制的阐明和临床诊疗提供有力帮助。结论:量子点荧光标记材料具有信号稳定、标记简便、检测灵敏的优点,在生物成像中具有良好的应用前景。

OBJECTIVE： To introduce the characteristics and application of quantum dots in biological imaging, and look ahead the future of biological imaging. DATA SOURCES： A computer-based search of PubMed database was undertaken for the articles related to quantum dots in biological imaging from January 1998 to April 2007, with the key words of ＂quantum dots, biological imaging, bioconjugates, fluorescence imaging＂ in English. STUDY SELECTION： The data were selected primarily. Inclusive criteria： ①characteristics and surface decoration of quantum dots.②application of quantum dots in biological imaging. Exclusive criteria： repetitive research. DATA EXTRACTION： There were 31 literatures in accordance with the above-mentioned criteria, among them, 14 were about characteristics and surface decoration of quantum dots, and 17 were about application of quantum dots in biological imaging. DATA SYNTHESIS： Quantum dots are a new kind of nano-fluorescent material, and have become popular as a highlight and stable fluorescent imaging probes in biological imaging due to the combination of quantum dots and biomoiecule. Compared with organic fluorescein and fluorescin, quantum dots have unique optical properties and can be used for studying gene or protein in cell, tissue, living animals, and drug target as well, which will all assist to interpret the disease mechanism and clinical diagnosis and treatment. CONCLUSION： Quantum dots fluorescence-labeled materials are stable in signals, convenient in labeling, and sensitive in detection, thus they are favorably applicable in biological imaging.