金钱白花蛇药酒对佐剂性足跖肿胀大鼠的干预效应

Interventional effect of Jinqianbaihuashe medicated wine on swollen foot pad induced by adjuvant in rats

目的:观察金钱白花蛇药酒对急性炎症模型的影响,探讨其作用特点。方法:实验于2002-02/05在河南中医学院动物实验中心完成。①将40只昆明小鼠随机数字表法分为大、小剂量的金钱白花蛇药酒组(分别灌服9,4.5g/kg金钱白花蛇药酒混悬液),枉痹冲剂组(枉痹冲剂混悬液4.5g/kg)和生理盐水组(同体积的生理盐水),每组10只。灌服给药1次/d,连续5d,于最后1次给药后1h,每只鼠右耳涂二甲苯,麻醉后处死小鼠,剪下双耳,用打孔器打下双耳耳片,迅速用分析天平称质量。并计算肿胀度。肿胀度(mg)=右耳质量(mg)-左耳质量(mg)。②将32只SD大鼠随机数字表法分为大、小剂量金钱白花蛇药酒组(9,4.5g/kg),枉痹冲剂组(4.5g/kg)及生理盐水组,每组8只。灌服给药1次/d,连续给药5d,最后1次给药后30min,在每只鼠左、右后足跖皮下注射新配制的100g/L新鲜鸡蛋清液0.1mL,分别于给蛋清后30,60,120,240,360min在足跖测定仪再次测大鼠左右后足跖体积,并计算足跖肿胀率。③将56只SD大鼠随机数字表法分为大、小剂量金钱白花蛇药酒预防组(9,4.5g/kg),大、小剂量金钱白花蛇药酒治疗组(9,4.5g/kg),枉痹冲剂预防组(4.5g/kg),枉痹冲剂治疗组(4.5g/kg)及佐剂模型组(0.5mL佐剂),每组8只。大鼠于乙醚麻醉下,以Freud’s完全佐剂0.05mL注射于右后足垫内,注射佐剂当日即开始给药为枉痹冲剂预防组,第8天开始给药为枉痹冲剂治疗组,1次/d,注射佐剂前及后3h以及间隔一定日期各测左右足趾肿胀程度1次,并观察鼠耳部红斑、尾部结节等出现情况,连续到21d,停药后继续观察3d,第24天麻醉后处死动物称取脏器。结果:纳入雄性昆明种小鼠40只、SD雄性大鼠88只,均进入结果分析。①金钱白花蛇药酒对小鼠耳壳二甲苯致肿的影响:与生理盐水组比较,大剂量金钱白花蛇药酒组和枉痹冲剂组均可显著抑制二甲苯所致小鼠耳壳急性肿胀(P<0.01),小剂量金钱白花蛇药酒组亦可明显抑制二甲苯所致小鼠耳壳急性肿胀(P<0.05)。②金钱白花蛇药酒对大鼠蛋清性足跖肿胀的影响:与生理盐水组比较,大、小剂量金钱白花蛇药酒组和枉痹冲剂组在30～240min均可显著抑制蛋清所致的大鼠足跖肿胀,使肿胀率显著降低(P<0.01);在360min,各给药组亦可明显抑制蛋清所致的大鼠足跖肿胀,使肿胀率明显降低(P<0.05);以大剂量组作用为优。③金钱白花蛇药酒对大鼠佐剂性关节炎的影响:与佐剂模型组比较,金钱白花蛇药酒预防组明显抑制注射局部的早期炎症反应和再度肿胀,又明显抑制另侧迟发性超敏反应性足跖肿胀,作用强度大体上和枉痹冲剂相似(P<0.01,P<0.05)。金钱白花蛇药酒治疗性给药可使注射佐剂侧足肿胀明显消退,并使再度肿胀的程度明显下降,对另侧迟发性超敏反应性足垫肿胀亦有明显的抑制作用(P<0.01,P<0.05)。结论:金钱白花蛇药酒有较好的抗炎作用。

AIM： To discuss the effect and action characteristics of Jinqianbaihuashe （JBS, Bungarus Parvus） medicated wine on the model of acute inflammation. METHODS： The experiment was performed in the animal experiment center of Henan University of Chinese Medicine from February to May 2002. ①Forty Kunming mice were randomly divided into large and small dose JBS medicated wine groups, which were gastncally infused with 9 and 4.5 g/kg JBS medicated wine suspension, Wangbi Granules group （4.5 g/kg） and normal saline group （4.5 g/kg） once a day for 5 days with 10 animals in each group. One hour after administration for the last time, all mice were executed under aesthesia after their right ear was embrecated with dimethylbenzene. The two ears were cut off, then two pieces of ears were removed by the stiletto and weighed immediately to count the degree of tumefaction （mg）： weight of right ear （mg）-weight of left ear （mg）. ②Thirty-two SD rats were randomly divided into large and small dose JBS medicated wine group, which were gastrically infused with 9 and 4.5 g/kg JBS medicated wine suspension, Wangbi Granules group （4.5 g/kg） and normal saline group （4.5 g/kg） once a day for 5 days with 8 animals in each group. Thirty minutes after administration for the last time, 0.1 mL fresh egg white liquid （100 g/L） was subcutaneously injected into voix pedis of rats. The volume of voix pedis was measured 30, 60, 120, 240, and 360 minutes after injection to count the degree of tumefaction. ③Fifty-six SD rats were randomly divided into large and small dose JBS medicated wine prevention groups （9, 4.5 g/kg）, large and small dose JBS medicated wine treatment group （9, 4.5 g/kg）, Wangbi Granules prevention group （4.5 g/kg）, Wangbi Granules treatment groups （4.5 g/kg） and adjuvant model group （0.5 mL） with 8 animals in each group. 0.05 mL Freud＇s complete adjuvant was injected into the right posterior foot pad of rats after anesthetized by aether. The Wangbi Granules prevention and treatment groups were administrated from the first and eighth days of adjuvant injection, once a day. The swelling degree was detected before and 3 hours and some days after adjuvant injection and ear red erythema and stern node were observed until the 21^st day. After withdrawing medicine, the animals were continued to observe for 3 days, and then their organs were harvested after executing them under anesthesia. RESULTS： Totally 40 male Kunming mice and 88 male SD rats were involved in the result analysis. ①Compared with normal saline group, Wangbi Granules and large dose JBS medicated wine could remarkably relieve the dimethylbenzene-induced tumefaction of ear （P 〈 0.01）, and small dose JBS medicated wine also lessened the tumefaction （P 〈 0.05）.②Compared with normal saline group, large and small dose JBS medicated wine and Wangbi Granules remarkably reduced the tumefaction of rats＇ voix pedis caused by egg white within 30 to 360 minutes （P 〈 0.01, P 〈 0.05）, especially large dose JBS medicated wine group. ③Compared with adjuvant model group, JBS medicated wine prevention group remarkably relieved the early inflammation and re-swelling of injected part and significantly reduce the late hypersensitive foot pad swelling, which was similar to the effect of Wangbi Granules （P 〈 0.01, P 〈 0.05）. The swelling of adjuvant injected part in JBS medicated wine treatment group was greatly lessened, and the inflammation of injected part was reduced. Moreover, late hypersensitive foot pad swelling was obviously inhibited （P 〈 0.01, P 〈 0.05）. CONCLUSION： JBS medicated wine displays good effect of anti-inflammation.