胶原性关节炎大鼠滑膜组织细胞因子基质金属蛋白酶3和组织金属蛋白酶抑制剂1表达的变化

Expressions of matrix metalloproteinase-3 and tissue inhibitor of metalloproteinase-1 in the synovium of collagen-induced arthritic rats

目的:观察Ⅱ型胶原诱导的实验性关节炎动物模型组织细胞因子基质金属蛋白酶3和组织金属蛋白酶抑制剂1表达的变化,探讨基质金属蛋白酶3和组织金属蛋白酶抑制剂1在类风湿关节炎发病机制中的作用。方法:实验于2005-09/2006-11在白求恩国际和平医院中心实验室完成。①实验分组:健康雄性Wistar大鼠60只,体质量(100±20)g,随机选出50只以备造模,另外10只作为对照组。在造模成功的大鼠中再随机选出10只作为模型组。②实验方法:采用10mg牛Ⅱ型胶原与100mg弗氏不完全佐剂和60mg卡介苗充分研磨后,以每支每点0.1～0.2mL(1g/L)从大鼠背部尾根部多点皮下注射,于10d后按上述方法和剂量再次重复免疫和制作大鼠胶原性关节炎模型。③实验评估:初次免疫后7周,剥离膝关节滑膜,进行苏木精-伊红染色光镜下观察;切除另侧膝关节滑膜,免疫组织化学染色后,用Metamorph图像分析系统进行检测,计算每个视野基质金属蛋白酶3和组织金属蛋白酶抑制剂1的阳性面积比,以其作为阳性反应细胞密度。结果:纳入大鼠60只,20只进入结果分析。模型组大鼠关节组织中的基质金属蛋白酶3阳性密度为0.75±0.19,明显高于对照组,差异有显著性意义(P<0.01);组织金属蛋白酶抑制剂1的表达阳性密度为0.48±0.17,与对照组0.78±0.21比较明显降低,差异有显著性意义(P<0.01)。结论:细胞因子基质金属蛋白酶3和组织金属蛋白酶抑制剂1表达的变化与胶原性关节炎大鼠的发病相关。

AIM： To observe the expression of matrix metalloproteinase-3 （MMP-3） and tissue inhibitor of metalloproteinase-1 （TIMP-1） of synovium in type Ⅱ collagen-induced experimental arthritic rats and investigate the effect of MMP-3 and TIMP-1 in the pathogenesis of rheumatoid arthritis （RA）. METHODS： The experiment was conducted in the Central Laboratory of Bethune International Peach Hospital from September 2005 to November 2006. ①Fifty of 60 healthy male Wistar rats of （100±20） g were selected for the preparation of modeling, and the other 10 served as controls. Among the successfully modeled rats, 10 were randomly selected as model group. ②10 mg cattle type Ⅱ collagen, 100 mg incomplete Freund adjuvant and 60 mg bacilli calmette-cjuerin were grinded together, and then 0.1-0.2 mL （1 g/L） every point was intradermally injected at the back and root of tails of rats with multiple points. Re-immunization was carded out according to above mentioned method and dosage. ③Seven week after the first immunization, synovium of knee joint was separated and observed under light microscope by HE dying; the synovium of the other knee joint was cut off and the expression of MMP-3 and TIMP-1 was detected by immunohistochemical method and analyzed with Metamorph image analysis system to calculate the positive area ratio in each visual field, which equaled to the density of positive cells. RESULTS： Of the 60 rats, 20 were involved in the result anaiysis. The positive density of MMP-3 in synovial tissues of model group （0.75±0.19） was significantly higher than control group （P 〈 0.01）; the positive density of TIMP-1 in model group was （0.48±0.17）, which was remarkably decreased compared with control group （0.78±0.21, P 〈 0.01）. CONCLUSION： Cytokines MMP-3 and TIMP-1 are correlated with the pathogenesis of arthritis induced by collagen.