人参皂甙Rg3在大鼠角膜新生血管中的作用研究

Effect of ginsenoside Rg3 on cautery-induced rat corneal neovascularization

目的研究人参皂甙Rg3对大鼠角膜新生血管(CNV)的作用。方法建立角膜碱烧伤后CNV模型,SD大鼠随机分为对照组和0.1、0.2、0.5mg/L人参皂甙Rg3治疗组。显微镜下观察各组CNV生长情况并计算CNV面积;免疫组织化学和半定量逆转录聚合酶链式反应(RT-PCR)检测各组角膜内血管内皮生长因子(VEGF)在不同时间点的表达变化。结果人参皂甙Rg3不同质量浓度组均能显著抑制CNV生长,0.5mg/L人参皂甙Rg3组抑制作用最显著(P<0.05)。结论人参皂甙Rg3对大鼠角膜碱烧伤后CNV的生长有明显抑制作用,其作用机制与抑制VEGF表达有关。

Objective Corneal neovascularization （CNV） is a severe complication induced by various pathogenetic factors. Ginsenoside Rg3 is an active ingredient element from ginsen. The purpose of this paper was to evaluate the antiangiogenesis of ginsenoside Rg3 in rat corneal neovascularization. Methods Inflammatory corneal neovascularization was induced by cautery with alkali in 64 eyes of 64 Sprague-Dawley rats. Animals were randomly divided into 4 groups. 0. 1,0.2 and 0.5 mg/L of ginsenoside Rg3 was topically administrated after cautery of corneas in B, C, D group respectively, and the normal salt solution was used in A group as control. The area of corneal neovascularization was detected under the slit lamp microscope. Immnuohistochemistry and relative quantitative reverse transcription polymerase chain reaction were used to investigate the distribution of vascular endothelial growth factor （VEGF） in rat cornea at different time. Results The growth of corneal neovascularization could be inhibited significantly in different ginsenoside Rg3 groups. 0.5 mg/L of ginsenoside Rg3 showed the strongest efficiency in treatment groups. Compared to the control group, the area of corneal neovascularization was significantly reduced in 0.5 mg/L ginsenoside Rg3 group on 1 day,4,7,14 days （P 〈 0.05）. Immunohistochemistry showed that the positive cell number of VEGF in ginsenoside Rg3 group were 56.35±8.25,39.34 ±3.35,15.45 ±3.69,8.89 ± 1.56 on 1 day ,4,7,14 days, and those of control group were 27.89 ± 2.36,18.23 ± 1.24,67.41 ± 4.89,42.24 ± 5.21 , showing a significant difference between two groups at various time points（ P 〈0.05 ）. The expression of VEGF mRNA in 0.5mg/L ginsenoside Rg3 group （35 ± 1 ） was lower significantly than that of the control group（ 114 ± 12,P 〈 0.05 ）. Conclusion Ginsenoside Rg3 can effectively inhibit the cautery-induced corneal neovascularization through the inhibition of VEGF.