摘要
研究和发展了一种两维荧光光谱测量及成像技术,讨论获得两维空间离散点的最佳光谱分辨率的方法.该技术以棱镜色散原理为基础,利用微透镜阵列实现对样品的离散化照明,充分发挥光学系统固有的并行处理能力,实现了在一个面阵探测器上同时记录两维空间的荧光光谱图像.与逐点扫描方法相比,该技术的扫描效率随激发点数线性提高,具有快速测量、高空间和高光谱分辨的特点,可用于生物医学领域.
A 2D fluorescence spectroscopy technique was proposed, and a design method for optimal spectral resolution of 2D discrete points was discussed. This new technique was based on prism dispersion theorv. It emploied a microlens array for discrete illumination on the samples. With the intrinsically parallel processing capability of the optical elements and system, the 2D spectrally resolved images can be recorded with one array detec, tor at the same time. Compared with the pixel-by-pixel laser scanning technique, the scanning efficiency of this technique is improved in linear proportion to the excitation spots. Due to its capability of quick measurement and high spatial and spectral reso- lutions, this technique is expected to have wide applications in biomedicine field.
出处
《深圳大学学报（理工版）》
EI
CAS
北大核心
2007年第3期252-256,共5页
Journal of Shenzhen University(Science and Engineering)
基金
国家自然科学基金资助项目(60627003)
深圳大学科研启动基金资助项目(200728)
关键词
荧光光谱技术
光谱分辨率
棱镜
微透镜阵列
生物医学
fluorescence spectroscopy technique
spectral resolution
prism
microlens array
biomedicine
