青蒿琥酯诱导HL-60细胞凋亡的研究

Apoptosis of HL-60 Cells Induced by Artesunate In Vitro

本研究旨在研究青蒿琥酯体外诱导HL-60细胞凋亡及其过程中Bcl-2与ICAD蛋白表达的变化。采用MTT法测定青蒿琥酯对HL-60细胞的生长抑制作用;用光学显微镜检、琼脂糖凝胶电泳、流式细胞术观察和检测青蒿琥酯诱导HL-60细胞的凋亡;应用蛋白印迹法检测青蒿琥酯诱导HL-60细胞凋亡过程中Bcl-2与ICAD的表达变化情况。结果表明:青蒿琥酯对HL-60细胞有明显的生长抑制作用,并呈时间、剂量依赖性。48小时IC50值为18.33μg/ml,其增殖抑制作用与诱导细胞凋亡有关;Bcl-2和ICAD在HL-60细胞中均有表达,且随药物浓度的增高表达量逐渐降低。结论:青蒿琥酯可诱导HL-60细胞凋亡,Bcl-2和ICAD在青蒿琥酯诱导HL-60细胞凋亡的信号传导途径中可能是重要的调控因子。

The study was aimed to investigate the effect of artesunate （ART） on the apoptosis of HL-60 cells in vitro and the expression of Bcl-2 and ICAD in the process of apoptosis induced by ART. The inhibition of ART on HL-60 cells were evaluated by means of MTT assay; cell apoptosis was detected by light microscopy, agarose gel electrophoresis, flow cytometry; Western blot was used to analyze the expression of Bcl-2 and ICAD in cells during apoptosis induced by ART. The results showed that ART could significantly inhibit the proliferation of HL-60 cells in time-and dose-dependent manner. After treating HL-60 ceils with ART for 48 hours, the IC50 values was 18. 33μg/ml and its inhibition effect contributed to the induced apoptosis. Bcl-2 and ICAD proteins both all expressed in HL-60 cells, the level of expression declined as concentration increased. It is concluded that artesunate may induce apoptosis of HL-60 cells in vitro, Bcl-2 and ICAD may be an important control factor in the signal transduction pathway of ART-induced apoptosis.