中药复方君子汤联合环孢霉素A逆转白血病细胞株K562/VCR耐药性的实验研究

Synergistic Reversal Effect of Chinese Medicine Compound FFJZ Combined with Cyclosporine A on Multidrug Resistance of Leukemia K562/VCR Cell Line

本研究观察中药复方君子汤(FFJZ)联合环孢霉素A(cyclosporine A,CsA)逆转白血病耐药细胞系K562/VCR细胞耐药性的效果,以便寻找有效的联合逆转剂。采用MTT法、流式细胞术研究FFJZ联合CsA逆转白血病耐药细胞系K562/VCR耐药性的作用。结果表明:FFJZ联合CsA在体外对K562/VCR细胞的耐药性有明显的逆转作用(p<0.01),能提高K562/VCR细胞对阿霉素(ADM)的敏感性,且在药物有效浓度范围内对细胞本身无毒性作用。FFJZ联合CsA对K562/VCR细胞的P-gp表达的阳性率无明显影响。结论:复方君子汤联合环孢霉素A可能成为治疗白血病多药耐药的安全有效的耐药逆转药物。

The study was purposed to investigate the synergistic reversal effect of Chinese medicine compound FFJZ in combination with cyclosporine A（CsA） on the multidrug resistance （MDR） of human leukemia K562/VCR cell line, as to search effective combination of MDR modulators. MTT （methyl-thazol-tetrazolinum） assay were used to determine the cytotoic and reversal effects on K562/VCR cell line, FCM （ flow cytometry） was used to assess the intracellular adriamycin （ADM） concentration and the expression of P-gp in cells. The rasults showed that the FFJZ in combination with CsA could reverse the drug-resistance of K562/VCR cells and increase the sensitivity K562/VCR cells to adriamycin. They had not the toxic effect on the K562/VCR cells in effective dose and no significant influence on P-gp positive rate of the K562/VCR cells. It is concluded that the FFJZ in combination with CsA may become a safe and effective multidrug resistance-reversing agent with low toxicity in leukemia chemotherapy.