力竭性游泳对大鼠胰岛 A、B 细胞形态学变化的影响——免疫组化及原位分子杂交结果分析

Effects of Exhaustive Swimming on Morphology of Islet A and B cells in Mice

采用免疫组化及原位分子杂交技术，本文研究了负重力竭性游泳对大鼠胰岛Ａ、Ｂ细胞分泌功能的影响。主要结果发现：力竭状态下，①大鼠胰岛Ｉｎｓ－ＩＲ细胞呈色反应强度增加，而Ｇｌｕ－ＩＲ细胞呈色反应强度降低，说明Ｂ细胞Ｉｎｓ释放被抑制，Ａ细胞Ｇｌｕ释放增多。②大鼠胰岛Ｂ细胞内Ｐｒｏ－ＩｎｓｍＲＮＡ杂交信号明显减弱，说明Ｐｒｏ－Ｉｎｓ－ｍＲＮＡ转录受到抑制，Ｉｎｓ合成受抑。③Ｉｎｓ－ＩＲ和ＩＡＰＰ－ＩＲ细胞未呈现相同程度的呈色反应变化，提示Ｂ细胞内存在着含ＩＡＰＰ／Ｉｎｓ不同比率的分泌颗粒，可随机体的机能状态需要而分别释放。

With immunohistochemical PAP method and technique of hybridization in situ,this study investigated the effects of exhaustive swimming on secretion function of islet A and B cells in mice.Results showed that:in exhausted mice,1,color reaction intensity of islet Ins IR cells enhanced and Glu IR cells reduced as compared with controls,suggesting the suppression of B cell releasing and increase of A cell secretion.2,Pro-Ins mRNA hybrid signal significantly weakened in B cells showing inhibition of Pro-Ins mRNA transcription and Ins synthesis 3,IAPP IR cells did not show the same color reaction intensity as Ins-IR cell,indicationg that IAPP was not secreted as equal as Ins.It suggested that the ratio of IAPP/Ins grains in B cells was different and the releases of IAPP and Ins may have seperate way according to the demand of the body.