摘要
采用水培实验方法研究了稀土元素La(Ⅲ)对紫外辐射(UV-B:0.15Wm-2,0.45Wm-2)胁迫下大豆(Glycine max)幼苗的δ-氨基乙酰丙酸(ALA)、胆色素原(PBG)、原叶绿素(酸)(Pchl)、原卟啉IX(Proto-IX)、镁原卟啉(Mg-Proto)等5种叶绿素(Chl)合成中间产物含量及Chl降解关键酶-叶绿素酶(Chlase)活性的影响,从叶绿素的生物合成及降解角度诠释La(Ⅲ)对UV-B辐射胁迫下大豆幼苗叶绿素含量影响的机理。实验结果表明:La(Ⅲ)具有提高叶绿素生物合成中间产物——ALA(δ-氨基乙酰丙酸)、PBG(胆色素原)、Proto-IX(原卟啉IX)、Mg-Proto(镁原卟啉)含量的作用,促进Pchl(原叶绿素)转化为叶绿素,进而缓解UV-B辐射胁迫下中间产物PBG、Proto-IX和Mg-Proto的形成,使叶绿素的生物合成受阻,受阻位点为ALA→PBG。
Effect of La on the chlorophyll content of Soybean （Glycine max） seedling under elevated uhraviolet-B radiation （UV-B , 280 -320 nm） was studied. When seedling was pretreated by La （ Ⅲ ）, the content of the interim outcomes of the chlorophyll such as δ-amino-laevulie acid （ALA）, proto bile pigment （ PBG）, proto-porphyrin-Ⅸ （Proto-Ⅸ） and magnesium proto-porphyrin （Mg-Proto） increased, and the process from protochlorophyll to chlorophyll was accelerated. The La （ Ⅲ ） promoted the protective effect of plant against UV-B stress through delaying the form of the PBG, Proto-Ⅸ,Mg-Proto and ALA. Therefore, the chlorophyll biosynthesis was suffocated under the condition of UV-B stress. The restrained location was from ALA to PBG.
出处
《中国农业气象》
CSCD
2007年第3期285-288,共4页
Chinese Journal of Agrometeorology
基金
国家自然科学基金(30570323)
江苏省科技厅高新技术基金(GB2005040)
