蚕丝蛋白膜法在粪便隐血试验中的应用评价

Applied Evaluation of Silkworm-fibrin Membrance in the Testing Method for Negative Blood Excrements

目的通过几种隐血试验方法的比较,了解蚕丝蛋白膜法检测大便隐血试验方法的可靠性。方法以世界卫生组织和世界胃肠镜检查协会推荐作为粪便隐血试验较为确认的胶体金免疫法为标准,同时用蚕丝蛋白膜法(自制)、免疫法(便隐血胶体金)、化学法(纸片法和联苯胺法)进行灵敏度、特异性、抗干扰和盲测试验。结果蚕丝蛋白膜法和便隐血胶体金法的灵敏度为0.1μg Hb/ml。特异性:只与人血红蛋白反应,与其它动物血不反应;与胶体金法盲测结果无明显差异(P>0.05),与化学法(纸片法和联苯胺法)相比有非常显著性差异(P<0.01)。90例经便隐血胶体金法确证为阳性的标本,蚕丝蛋白膜法90例结果全部呈阳性,符合率100%。结论经临床应用结果证明,此法简便快速,灵敏度高,特异性强,适合临床应用。

Objective To compare with several negative blood exprerimental methods to find out the reliablity of the negative blood method of the test for excrements by silkworm-fibrin membrance, Methods The negative blood test which is reccommended by the WHO and The World Test for Stomach Association were confirmed as the standard of was immuno- gold assay as well as having the experimental test for sensitivity,specificity and anti-disturbance by the methods of silk- worm-fibrin membrance （made by own） and immunogold assay （for negative blood）,chemical method （paper test and benzidine test）. Results The sensitivity of silkworm-fibrin membrance test and negative blood immunogold assay was 0. 1μg Hb/ml. The specificity ：just reflection of human＇s blood ,not of other animals＇ blood. Meanwhile,there was no obvious difference of results with the immunogold assay test＇s （P〉0.05）. Compared with the chemical method ,there existed very obvious difference（P〈0.01）. The 90 cases were confirmed as the positive sample by negative blood immunogold assay and another 90 cases tested by silkworm-fibron membrance were presented positive, which reached 100%. Conclusion Through the prove of clinic applied result,this method is especially applied to the clinic apply with the simble and rapid sensitivity as well as high and intense specificity.