rhG-CSF 和氟康唑联合应用对粒细胞减少小鼠念珠菌肺感染的疗效

COMBINED EFFECT OF rhG CSF AND FLUCONAZOLE ON PULMONARY CANDIDIASIS IN NEUTROPENIC MICE

为了研究人类重组粒细胞集落刺激因子（ｒｈＧ－ＣＳＦ）对中性粒细胞减少小鼠白色念珠菌肺感染的预防和治疗作用，腹腔注射环磷酰胺的小鼠被分成４组：①对照组，气管内注射白色念珠菌；②感染前皮下注射ｒｈＧ－ＣＳＦ；③感染后１２ｈ腹腔注射氟康唑；④ｒｈＧ－ＣＳＦ和氟康唑联合应用。结果显示注射白色念珠菌前４ｄ，皮下注射ｒｈＧ－ＣＳＦ１００μｇｋｇ－１ｄ－１对中性粒细胞减少合并白色念珠菌肺感染小鼠有明显的保护作用；白色念珠菌感染后２４ｈ，肺内中性粒细胞数较对照组明显增多；ｒｈＧ－ＣＳＦ和氟康唑联合应用较单独应用疗效好。

To study the prophylactic and therapeutic effects of human recombinant granulocyte colony stimulating factor (rhG CSF) on pulmonary candidiasis in neutropenic mice, the mice were injected intraperitoneally with cyclophosphamide (200mg·kg -1 ) and divided into four groups: ①vehicle, only intratracheal injection with 5×10 6 candida albicans; ②subcutaneous injection with rhG CSF 4,2 or 0 day(s) before the infection; ③intraperitoneal injection with fluconazole 12h after the infection; ④injection with a combination of rhG CSF and fluconazole. The results showed that rhG CSF, especially with 100 μg·kg -1 ·d -1 and four days before injection of candida albicans, had significant protection against pulmonary candidiasis in neutropenic mice. The infiltration of polymorphonuclear leukocytes (PMNs) to the infected lung at 24h after injection of candida albicans was more remarkable in the rhG CSF treated mice than that in the controlled mice. Combined treatment with rhG CSF and fluconazole was more effective than the treatment with either agent alone.