大白鼠小肠缺血再灌注后氧自由基所致肺损伤

The Role of Reactive Oxygen Species on Pulmonary Injury After Intestinal Ischemia Reperfusion

目的:探讨肠缺血再灌注后肺损伤的病理机制。方法:对15只大白鼠小肠缺血再灌注模型进行血和肺组织脂质过氧化物测定,并观察其病理改变。结果:大白鼠小肠缺血后再灌注,血脂质过氧化物明显升高,特别是缺血60分钟组(P<0.01);同时肺组织匀浆内脂质过氧化物明显高于对照组(P<0.01),肺病理改变为组织充血,炎症细胞浸润,肺泡不张。结论:小肠缺血再灌注后氧自由基的产生是生命器官损伤的主要原因,其中肺损伤是并发呼吸衰竭的基础。

Objective:To study the role of reactive oxygen species on pulmonary injury after intestinal ischemia/reperfusion(I/R).Methods:The lipid peroxides(LP)of blood and lung tissue of 15 rats after intestinal I/R were measured.The rats were divided into three groups.Group A was controls,group B underwent 30 minutes total mesenteric occlusion and group C 60 minutes occlusion.Results:The serum LP concentration after I/R injury were higher than that of control (P<0.01),especially in group C.The LP level in lung homogenate also significantly in creased after intestinal I/R injury(P<0.01).At the same time,the morphological examination of the lungs showed pneumochysis and pneumonorrhagia.This observation agreed with the results of LP in serum and the lung homogenate.Conclusions:The LP after intestinal I/R plays certain role for local and systemic injury,and the reactive oxygen species plays an important role in pulmonary in- jury.It may be the cause of respiratory failure in this kind of diseases.