移植脾淋巴细胞功能及蛋白质合成分析

Alteration of Lymphocyte Functions and Protein Synthesis in the Autologous Transplanted Spleen-

目的:探讨自体移植脾组织能否降低 OPSI 的发病率。方法:对再生脾本身进行研究,32只 SD 雌性幼龄大鼠分为两组,对照组16只,自体脾组织移植组16只。术后22周检测脾细胞对羊红细胞(SRBC)的抗体反应及对有丝分裂原增殖反应,并用 SDS-聚丙烯酰胺凝胶电泳分析其蛋白组成。结果显示:移植组脾细胞对 SRBC 抗体生成无明显改变(P<0.01),T 细胞对刀豆素 A增殖反应明显减弱,而 B 细胞对脂多糖反应正常。说明移植睥组织经再生后,B 细胞数量和功能正常,而 T 细胞功能发生了改变。蛋白电泳显示自体移植脾细胞与正常脾细胞相比,能产生更明显的19000带,而16000、29000带缺失。结论:再生脾与正常脾组织在分子水平和功能上存在差异,脾切除后仅行自体脾组织移植还不能达到预防 OPSI 的目的。

Objective:To investigate the functions of autotransplanted spleen.Methods: Thirty-two female SD rats were equally divided into 2 groups:the sham-operated group and the spleen autotransplantation group.The antibody responses of spleen cells to sheep red blood cells (SRBC)and the proliferation rate induced by mitogen stimulation were performed.Protein compo- sition of splenic tissue was analyzed by SDS-polyacrylamide gelelectrophoresis(SDS-PAGE)22 weeks after operation.Results:Antibody responses to SRBC were not different from the controls and the autotransplants(P<0.01).The stimulation index by ConA for T cell decreased remark- ably and the proliferation of B cells after LPS stimulation in transplants was normal.These find- ings indicated a normal development of B cell and a altered development of T cell in the transpants.Electrophoresis showed mainly two protein groups with a molecular weight of 16000 and 29000 produced in normally detectable concentration in the spleen cells but not in autotrans- plant cells.Proteins with a molecular weight of 19000 were over produced in the autotransplant. The findings showed a clear difference between the normal spleen and the transplant on the func- tional and molecular levels.Conclusions:Simple splenic autotransplants after splenectomy can not prevent overwhelming postsplenectomy infection.