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alkB基因在胃癌和萎缩性胃炎黏膜组织中的表达改变 被引量:2

Changes of alkB Gene Expression in Gastric Cancer and Atrophic Gastritis
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摘要 背景:DNA或RNA分子异常甲基化导致的抑癌基因沉默、突变或其他功能性障碍是胃癌发生的关键机制之一。最近研究发现alkB基因产物具有修复DNA和mRNA碱基异常甲基化、纠正基因突变、复制转录障碍等功能,但对其在胃癌及其癌前病变组织中的表达情况尚不了解。目的:探讨alkB基因在胃癌及其癌前病变组织中的表达改变。方法:收集11例胃癌、癌旁和远离癌灶正常黏膜组织以及107例慢性萎缩性胃炎和121例非萎缩性胃炎患者的内镜活检黏膜,应用基因表达谱芯片实验评估alkB基因在各组织中的表达。以逆转录聚合酶链反应(RT-PCR)检验上述结果。结果:与远离癌灶正常黏膜组织相比,alkB基因在胃癌中的表达降低(Ratio值为0.208);与非萎缩性胃炎黏膜组织相比,alkB基因在萎缩性胃炎黏膜组织中的表达降低(Ratio值为0.378);而alkB基因在癌旁和远离癌灶正常黏膜组织中的表达无显著差异(Ratio值为0.726)。结论:alkB基因在胃癌和萎缩性胃炎黏膜组织中的表达下调,可能参与了胃癌发生过程中基因甲基化紊乱的机制。alkB基因是有潜在研究价值的分子靶标。 Background: Transcriptional silencing of tumor suppressor genes, mutation and other functional disturbance produced by DNA or RNA hypermethylation play a crucial role in the development of gastric cancer. Recent studies have demonstrated that alkB gene product can repair the hypermethylation of DNA and mRNA, restore the biological function of mRNA and tRNA inactivated by methylation and replicate the transcriptional impairment, but its expression in gastric cancer and precancerous lesions has not been clarified. Aims: To appraise the changes of alkB gene expression in tissues of human gastric cancer and its precancerous lesions. Methods: Eleven cases of gastric cancer, paracancerous and noncancerous mucosal tissues, mucosal tissues from endoscopic biopsies of 107 patients with atrophic gastritis and 121 patients with non-atrophic gastritis were collected, cDNA microarray was used as a tool for assessment genes expression in different tissues, and the results were reexamined by reverse transcriptase polymerase chain reaction (RT-PCR). Results: Compared with non-cancerous mucosal tissue, the expression of alkB gene were down-regulated in gastric cancer (Ratio value was 0.208); compared with non-atrophic gastritis mucosal tissue, the expression of alkB gene was lower in atrophic gastritis mucosal tissue (Ratio value was 0.378); but no significant difference was found between paracancerous and non-cancerous mucosal tissues (Ratio value was 0.726). Conclusions: The down-regulation of alkB gene expression in gastric cancer and atrophic gastritis mucosal tissue may be involved in the mechanism of hypermethylation of tumor suppressor genes owing to development of gastric cancer, and is of potential value in the future study of the molecular target.
出处 《胃肠病学》 2007年第7期401-404,共4页 Chinese Journal of Gastroenterology
基金 国家自然科学基金项目(30570827) 上海市重点学科建设项目(Y0205)资助
关键词 基因 ALKB 胃肿瘤 胃炎 萎缩性 寡核苷酸序列分析 DNA甲基化 Gene, alkB Stomach Neoplasms Gastritis, Atrophic Oligonucleotide Array Sequence Analysis DNA Methylation
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  • 1Chang HW,Chow V,Lam KY,et al.Loss of E-cadherin expression resulting from promoter hypermethylation in oral tongue carcinoma and its prognostic significance.Cancer,2002,94 (2):386-392.
  • 2Si HX,Tsao SW,Lam KY,et al.E-cadherin expression is commonly downregulated by CpG island hypermethylation in esophageal carcinoma cells.Cancer Lett,2001,173 (1):71-78.
  • 3Graff JR,Herman JG,Lapidus RG,et al.E-cadherin expression is silenced by DNA hypermethylation in human breast and prostate carcinomas.Cancer Res,1995,55 (22):5195-5199.
  • 4Sakuma K,Chong JM,Sudo M,et al.High-density methylation of p14ARF and p16INK4A in Epstein-Barr virus-associated gastric carcinoma.Int J Cancer,2004,112 (2):273-278.
  • 5Koike H,Ichikawa D,Ikoma H,et al.Comparison of methylation-specific polymerase chain reaction (MSP)with reverse transcriptase-polymerase chain reaction (RTPCR) in peripheral blood of gastric cancer patients.J Surg Oncol,2004,87 (4):182-186.
  • 6Ichikawa D,Koike H,Ikoma H,et al.Detection of aberrant methylation as a tumor marker in serum of patients with gastric cancer.Anticancer Res,2004,24(4):2477-2481.
  • 7Kim TY,Jong HS,Jung Y,et al.DNA hypermethylation in gastric cancer.Aliment Pharmacol Ther,2004,20Suppl 1:131-142.
  • 8Pellisé M,Castells A,Ginès A,et al.Detection of lymph node micrometastases by gene promoter hypermethylation in samples obtained by endosonography-guided fineneedle aspiration biopsy.Clin Cancer Res,2004,10 (13):4444-4449.
  • 9Kang HC,Kim IJ,Park JH,et al.Promoter hypermethylation and silencing of CHFR mitotic stress checkpoint gene in human gastric cancers.Oncol Rep,2004,12 (1):129-133.
  • 10Sarbia M,Geddert H,Klump B,et al.Hypermethylation of tumor suppressor genes (p16INK4A,p14ARF and APC) in adenocarcinomas of the upper gastrointestinal tract.Int J Cancer,2004,111 (2):224-228.

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