紫外线照射剂量对九孔鲍胚胎及幼虫的影响

Effects of ultraviolet irradiations on the embryo and larvae of Haliotis diversicolor aquatilis

采用不同的紫外线照射剂量的海水诱导九孔鲍产卵，观察其对卵子的受精率、畸形率和孵化率的影响．紫外线照射剂量的配制方法是：用紫外线照射过的最大剂量的海水与新鲜海水混合配制出各种剂量的海水．实验把雌亲鲍分成7组，每组20只，分别用紫外线照射剂量为0（对照组）、450、600、750、900、1050和1200mW·h／dm^3的海水进行刺激产卵．试验重复3次．结果表明，紫外线照射剂量在1200mW·h／dm^3以内，对卵子的受精率影响不大；紫外线照射剂量在750mW·h／dm^3以内，对卵子的畸形率影响不大，但在900—1200mW·h／dm^3，对卵子的畸形率影响很大；紫外线照射剂量在600mW·h／dm^3以内，对卵子的孵化率没有什么影响，但在750—1200mW·h／dm^3，对卵子的孵化率影响很大．综合考虑卵子的受精率、畸形率和孵化率，九孔鲍催产的适宜的紫外线照射剂量应为450—750mW·h／dm^3。

Effects of different ultraviolet （UV） irradiation dosage on fecundity, abnormality and hatching rates of Haliotis diversicolor eggs were studied. The water used in the experiment were confected by blending the water treated with full UV irradiation with different percentage of untreated clean water. In this experiment the female matured Haliotis diversicolor aquatilis were divided into 7 groups, each with 20 pieces of Haliotis diversicolor aquatilis. UV irradiated sea water was used to acti- vate parent Haliotis diversicolor aquatilis. The ultraviolet radiation dosage were set to 0 （the controlled） ,450,600 ,750 ,900,1 050 and 1 200mW·h/dm^3. The experiment were triplicated. As resuits, it indicated that below 1 200mW·h/dm^3, the UV radiation affects little fecundity of the tested eggs ;below 750mW·h/dm^3, it affected slightly on the abnormality of eggs, between 900 and 1 200mW·h/dm^3,it leads to egg abnormality significantly. In the other hand, under 650mW·h/dm^3 it affects little on hatching rate;between 750 ~ 1 200mW·h/dm^3 the effects became significant. Optimizing the abnormality, fecundity and hatching rates, a proper UV irradiation on the eggs should be at the scale between 450 and 750mW·h/dm^3.