体外模拟CO_2气腔对MCF-7细胞黏附分子CD44v6表达及黏附力的影响

Influence of artificial CO_2 cavity on CD44v6 expression in MCF-7 cells and their adhesive ability

目的:探讨体外模拟的CO2气腔对MCF-7细胞黏附分子CD44v6表达和黏附力的影响。方法:体外建立人工气腔,MCF-7细胞在7mmHg(1mmHg=0.133KPa)的CO2分压下暴露l、2和4h;在处理后0、24、48和72h时,以流式细胞术测定CD44v6表达。用Vlodavsky[1]方法测定细胞黏附力。缺氧组选用0mmHg的N2(1h);对照组为细胞一直培养在37℃恒温培养箱。结果:1和2h的CO2组处理后0、24h时,CD44v6的表达显著高于对照组(P<0.05);4h的CO2组处理后0、24、48h时,CD44v6的表达显著高于对照组(P<0.05)。4h的CO2组处理后0h时细胞黏附力减小,而48h时反而大于对照组(P<0.05)。结论:体外模拟7mmHg的CO2气腔可使MCF-7细胞的CD44v6表达增高,并随着处理时间的增长有增高趋势。短时间的CO2气腔对细胞黏附力影响不显著。

Objective To investigate the influence of in vitro artificial CO2 cavity on adhesion molecule CD44v6 expression in MCF-7 cells, and on their adhesive ability. Methods An in vitro artificial CO2 cavity model was established. MCF-7 cells were exposed to CO2 under the pressure of 7 mmHg for 1,2 and 4 hours, respectively. CD44v6 expression was measured by flow cytometry 0, 24, 48 and 72 hours after CO2-insufflation. Adhesive ability of MCF-7 cells was evaluated by the assay of Vlodavsky. Hypoxia group was exposed to 0 mmHg N2 for 1 h, and the control group was exposed to 37℃ incubator only. Results Compared with those in the control group, the CD44v6 expression in the 1 and 2 hours treatment group were significantly elevated at 0 hour and after 24 hours （P〈0.01）, and those in the 4 hours treatment group were also elevated at 0 hour, and after 24, 48 hours （P〈0.01）. The adhesive ability of MCF-7 in the 4 hours treatment group was lower than that in the control group at 0 hour, but higher than that in the control group after 48 hours （P〈 0.05）. Conclusions The expression of adhesion molecule CD44v6 in MCF-7 cells is elevated after exposure to artificial CO2 cavity, which demonstrates a positive correlation with the duration of exposure. Short exposure to CO2 cavity could not significantly modulate the adhesive capacity of tumor cells.