山东实生板栗居群遗传多样性ISSR分析

Study on the Genetic Diversity of Natural Chestnut of Shandong by ISSR

采用ISSR分子标记技术对山东省内的10个板栗居群共279个个体的遗传多样性水平及居群遗传结构进行了研究。10个引物共检测到116个位点,其中101个位点为多态位点,占87.07%。POPGENE分析结果表明:板栗具有丰富的遗传变异(在物种水平上,He=0.2697,H0=0.3999;在居群水平上,PPL=64.58,He=0.2004,H0=0.3010)。Neis遗传多样性分析和AMOVA分析表明,各居群间产生了一定程度的遗传分化(GST=0.2414,FST=0.2224)。居群间一定程度的遗传分化可能是由于生境破坏和基因流的障碍(Nm=0.8743)引起。UPGMA聚类分析可知,临沭、莒南、郯城和费县4个居群优先聚成一支,而莱阳居群单独聚为一支。

The genetic diversity of 279 indivdiuals from 10 populations in Shandong Province was investigated using inter-simple sequence repeat （ISSR） markers. As a result, 116 bands were amplified by 10 informative and reliable primers, of which 101 were polymorphic loci. A relatively high level of genetic diversity was revealed ： PPL = 87.07, He = 0. 2697, H0 = 0. 3999 （ at the species level） ; PPL = 64.58, He = 0.2004, H0 = 0.3010 （at the population level）. A higher level of genetic differentiation was detected among populations with Nei＇s GST analysis and the analysis of molecular variance （AMOVA; GST = 0.2414, FST = 0.2224）. Habitat fragmentation and gene flow may result in genetic differentiation. UPGMA cluster analysis indicated that the four populations from Linshu, Junan, Tancheng and Feixian grouped together, whereas Laiyang populations clustered in an isolated clade. The results showed that a mixed mating system was possibly the main factor influencing the genetic structure of this species. These results, combined with other information about Castanea mollissima, may provide a valuable basis for proposing conservation strategies.