摘要
克隆测序短小芽孢杆菌(Bacillus pumilus)木聚糖酶基因,在同源建模所得三维结构的基础上寻找底物结合可能的活性口袋,用计算机模拟其与底物木聚糖的对接,预测酶与催化反应过程的关键氨基酸残基。所得的信息对木聚糖酶的定向改造有重要意义。
Bacillus pumilus xylanase was cloned and sequenced. Based on the tertiary structure that originated from homology modeling, the potential active pocket was searched and ligand-protein docking was performed using relative softwares. The information extracted from the molecular docking is analyzed; several amino acid residues might play a vital role in the xylanase catalytic reaction are obtained to instruct the further modification of xylanase directed-evolution.
出处
《生物工程学报》
CAS
CSCD
北大核心
2007年第4期715-718,共4页
Chinese Journal of Biotechnology
基金
国家973重点基础研究发展规划项目(No2007CB707804)
国家自然科学基金(No20046004
20676048)
福建省科技基金(No20031020)资助~~