谷氨酸对大鼠纹状体神经元活动的影响

Effects of L-glutamic Acid on the Activities of the Nerve Cell in Male Rat Striatum

目的:研究谷氨酸(L-glutamic acid,GLU)及受体阻断剂MK-801对雄性大鼠纹状体(striatum,STR)神经元自发放电活动的影响。方法:应用微电泳方法记录纹状体神经元自发放电活动。结果:微电泳GLU使84.13%STR神经元自发放电频率加快。在43个STR神经元中直接微电泳MK-801可使62.79%STR神经元自发放电频率降低。在微电泳GLU产生兴奋效应的33个STR神经元中,微电泳GLU期间给予MK-801拮抗GLU的兴奋效应,可使90.91%已对GLU产生兴奋效应的STR神经元放电频率减慢。结论:GLU对STR神经元起兴奋作用,但其兴奋作用可被MK-801所拮抗,当直接微电泳MK-801时,可使STR神经元产生抑制效应。研究提示抗谷氨酸作用的药物有抗帕金森病(Parkinson disease,PD)的作用。

The goal fect spontaneous discharges of the of the experiment is to study how nerve cells in male rat striaturM L-glutamic acid （GLU） and MK-801 afMethods. Microelectrodes were used to observe the effects of L-glutamic acid （GLU） and MK-801on STR neurons＇ spontaneous firing rates. Results： 84. 13% of the tested neurons were excited by GLU, With the increase of electrical currents of Microelectrophoresis, frequency of discharge of STR nerve cells accelerates. Among the 43 STR nerve cells, direct Microelectrophoresis MK-801 can lower the frequency of discharge of 62. 79% STR nerve cells. Of the 33 STR nerve cells on which Microlectrophoresis GLU has stimulating effect, in the period of Micro-electrophoresis GLU, MK-801 will counter-affect GLU＇ stimulating effect and as the result lower the discharging frequency of 90. 91% STR nerve cells which has been affected by GLU. Conclusions： GLU has the excitatory effect on STR nerve cells, which, however, may be antagonized, by MK-801; the direct Micro-electrophoresis MK-801 may result in inhibitory effect on the STR nerve cells. The study hints at the function of anti-glutamate medicine restraining Parkinson disease.