火鹤花离体培养无菌体系建立技术的研究

Study on the Building Technique of in vitro Sterility Culture System of Anthurium andraeanum

研究了火鹤花(Anthurium and raeanum)外植体、基本培养基类型、叶片生理部位、植物生长调节剂、光照条件等因素对其愈伤组织诱导的影响。结果表明,叶片的诱导率最高达97.5%,污染率最低,仅为4.9%;诱导愈伤组织发生率及发生速度依次为:1/2MS培养基>MS>N6>B5>White,幼叶>中龄叶>老叶,不含叶缘叶片>含叶缘叶片。愈伤组织在1/2MS+6-BA1.0 mg/L+NAA0.2 mg/L中长势最好;在1/2MS+6-BA2.0 mg/L+IBA0.1 mg/L中生长旺盛,不定芽健壮,可进一步生根培养;每日12h光照下愈伤组织发生率最高。

Effects of explant type, basic medium type, physiology position of leaves, different plant growth regulators and light on inducing callus were studied. The results indicated that the callus inductivity of leaves was the highest（97.5%） with the lowest contamination percentage（only 4.9 %）. The order of callus engendering proportion and rate indued in different mediums was 1/2MS〉MS〉N6 medium〉B5 medium〉White medium, while by different types of leaves was spire〉medium-year leaf〉old leaf and leaves no margin〉leaves with margin. Callus grew well when cultured by 1/2MS＋6-BA1.0 mg/L＋NAA0. 2 mg/L, and grew vigorously with robust bud cultured by 1/2MS＋6-BA2.0 mg/L＋IBA0.1 mg/L which could be cultured for rooting. Full light was not beneficial to callus induction, and the callus inducing rate was the highest when lighting 12 h/d.