红霉素抑制磨损颗粒诱发体内骨溶解的研究

An in vivo study of the inhibitory effect of erythromycin on wear particles induced osteolysis

[目的]通过小鼠体内磨损颗粒颅骨溶解模型研究红霉素(erythromycin,EM)抑制磨损颗粒诱发骨溶解的效果。[方法]24只8周龄的C57BL/J6雄性小鼠随机分为4组:聚甲基丙烯酸甲酯(polymethyl-methacrylate,PM-MA)组接受PMMA30 mg颗粒植入颅顶部,PMMA+2EM组接受30 mg PMMA颗粒植入加每天EM2 mg/kg腹腔内注射,PMMA+10EM组接受PMMA30 mg颗粒植入加每天EM10 mg/kg腹腔内注射,对照组接受假手术。7 d后取出颅骨进行病理学分析。[结果]颅骨破坏面积对照组为0.079 mm2±0.011 mm2,PMMA组0.335 mm2±0.129 mm2,PM-MA+2EM组0.094 mm2±0.019 mm2,PMMA+10EM组0.091 mm2±0.028 mm2。对照组颅骨实验区域内破骨细胞计数为5.3±1.0个,PMMA组为19.2±5.3个,PMMA+2EM组为6.6±1.1个,PMMA+10EM组为6.1±1.9个。与对照组相比,PMMA颗粒可以诱发骨溶解(P<0.001),而EM治疗可以抑制PMMA颗粒诱发的颅骨溶解(P<0.001),及破骨细胞生成(P<0.001)。[结论]EM可以抑制PMMA磨损颗粒诱发的骨溶解。

[ Objective ] In present study an in vivo wear particles induced mouse calvarial osteolysis model was used to investigate the inhibitory effect of erythromycin over wear particles induced osteolysis. [ Method ] Twenty-four male eight-week -old C57BL/J6 mice were allocated into 4 groups： PMMA group receiving 30 mg PMMA particles implantation onto the calvariae, PMMA ＋ 2EM group receiving 30 mg PMMA particles implantation and EM 2mg/kg ip injection, PMMA ＋ 10EM group receiving 30 mg PMMA particles implantation and EM 10mg/kg ip injection, control group receiving sham operation. Seven days later, mice calvariae were harvested for pathology analysis. [ Result] Middle suture area in the control group was 0.079 mm^2 ＋ 0.011 mm^2, in PMMA group 0.335 mm^2 ＋ 0. 129 mm^2. In PMMA ＋ 2EM group the area was 0. 094 mm^2 ＋ 0. 019 mm^2, PMMA ＋ 10EM group was 0.091 mm^2 ＋ 0. 028 mm^2. Osteoclasts number in osteolysis area in the control group were 5.3 ± 1.0, in PMMA group 19.2 ±5.3, PMMA ＋2EM group was 6.6 ± 1.1, PMMA ＋ 10EM group 6.1 ± 1.9. Compared with control group, PMMA particles induced significant osteolysis（ P 〈 0. 001 ） , erythromycin treatment effectively inhibited PMMA - particles - induced osteolysis （ P 〈 0.001 ） and osteoclastogenesis （ P 〈 0.001 ）. [ Conclusion ] Erythromycin can effectively inhibite wear particles induced in vivo calvarial osteolysis.