大花蕙兰(Cymbidium hybridum)试管苗基部切块诱导类原球茎研究

Micropropagation of Cymbidium hybridum by Inducement of PLB from Cuted Base of in vitro Plantlets

以大花蕙兰试管苗基部切块为外植体,以MS培养基为基本培养基,培养诱导类原球茎(PLB),研究不同激素配比以及外植体来源基因型对PLB诱导的影响。低水平的2,4-D和较高水平的6-BA对于试管苗基部切块诱导PLB具有显著的促进作用。当2,4-D浓度高于0.60 mg.L-1时,PLB诱导受到显著抑制。不同基因型有不同的最佳激素组合。NAA对特定基因型诱导PLB有效。以试管苗基部切块组织培养方式诱导大花蕙兰PLB是可行的,为转基因研究提供了植株再生技术基础。

PLB （ protocorm-like body ） is successfully induced from the cuted tissue of plantlet-base of Cymbidium hybridum in vitro, in a simulative manner like microtuber. The effects of different concentrations of 2,4-D, 6-BA, and NAA on the PLB formation of C. hybridum were investigated in different level of concentration. The results showed that the effects of 2,4-D and 6-BA on the frequency of PLB formation of C. hybridum differed extremely sig- nificantly. 2,4-D on lower level was a important stimulative to induce PLB, but the inducing of PLB would be restrained when the concentration of 2,4-D was above 0.60 mg · L^-1. The frequency of PLB formation increased basically with the increase of concentrations of 6-BA. The frequency of PLB formation was also related to the genotype, NAA was a important stimulative factor for some special genotype of C. hybridum. The experiment strategy to inducing PLB in simulative manner of bulb is feasible.