Ⅰ期尘肺患者血清白细胞介素12、白细胞介素12p70、白细胞介素10和白细胞介素18含量的变化

Change of serum interleukin-12,interleukin-12p70,interleukin-10 and interleukin-18 in pneumoconiosis stageⅠ

目的探讨血清白细胞介素12(IL-12)、白细胞介素12p70(IL-12p70)、白细胞介素10(IL-10)和白细胞介素18(IL-18)水平在Ⅰ期尘肺发生发展中的作用。方法采用双抗体夹心酶联免疫吸附测定(ELISA)法检测79例Ⅰ期尘肺患者(其中包括44例煤工尘肺患者和35例矽肺患者)、42例具有相同接尘史但未患尘肺的井下煤矿工人(接尘对照)及41例井上健康查体人员(正常对照)的血清IL-12、IL-12p70、IL-10和IL-18的含量。结果矽肺组IL-12和IL-10水平明显高于两对照组,组间差异有统计学意义(均P<0.05);煤工尘肺组IL-12和IL-10水平虽高于两对照组,但差异均无统计学意义(P>0.05);煤工尘肺组和矽肺组IL-12p70水平较两对照组均明显增高,差异具有统计学意义(P<0.01);而IL-18水平在各组间无明显改变(P>0.05)。煤工尘肺Ⅰ期、矽肺Ⅰ期及其对应的Ⅰ期合并高血压、冠心病和Ⅰ期并发慢性阻塞性肺疾病中4项指标水平比较,其差异无统计学意义(均P>0.05);煤工尘肺Ⅰ期和矽肺Ⅰ期患者不同接尘年限间比较,4项指标水平均未发现明显改变(P>0.05)。Ⅰ期尘肺患者血清IL-12、IL-12p70及IL-10三者之间呈正相关,而与IL-18均无相关性。结论Ⅰ期尘肺患者体内细胞因子网络紊乱可能与尘肺发病机制有关。

Objective To explore the change of serum interleukin-12 （IL-12）, interleukin- 12p70 （IL-12p70）, interleukin-10 （IL-10） and interleukin-18 （IL-18） and its possible role in the occurrence and development of pneumoeoniosis stage I. Methods Serum levels of IL-12,IL-12p70,IL-10 and IL-18 were determined in 79 patients with pneumoconiosis stage I ,including 44 cases of coal workers＂ pneumoconiosis（CWP group） and 35 cases of silicosis, besides,42 healthy coal workers under well and 41 healthy controls above well by a sandwich enzyme-linked immunosorbent assay （ELISA）. Results Compared with two control groups, serum levels of IL-12 and IL-10 in silicosis group were significantly higher, respectively（all P 〈0.05）; serum levels of IL-12 and IL-10 in CWP group were a little higher,but no significant difference（ P 〈0.05） ; serum levels of IL-12p70 in silicosis and CWP group were significantly higher（ P 〈 0.01）; however serum level of IL-18 had no significant difference among different groups （P 〉 0. 05）. No significant difference was observed in serum IL-12, IL-12pT0,IL-10 and IL-18 among CWP stage I , silicosis stage I and their complications（all P 〉0.05） ; There were no relationships between the four parameters and the duration of exposure to dust in CWP stage I and silicosis stage I （ P 〉0.05, respectively） ; There was positive correlation among serum levels of IL-12, IL-12p70 and IL-10 in pneumoconiosis stage I , however there was no correlation between IL-18 and other three parameters. Conclusion The pathogenesis of pneumoconiosis stage I was possibly associated with the apparent disturbance of the regulation networks of cytokines systems.