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PITX1和Pan-ras在肝癌细胞中的表达及意义 被引量：1

Expression of PITX1 and Pan-ras genes in primary hepatocellular carcinoma
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摘要 目的研究抑癌基因PITX1和其下游癌基因Pan-ras在正常胚肝细胞株L02,肝癌细胞株HepG2和SMMC-7721中的表达,探讨其在肝癌发生发展中的作用和关系。方法应用SABC免疫组织化学染色技术和western blot蛋白质印迹以及半定量RT-PCR检测L02、HepG2和SMMC-7721细胞株中PITX1和Pan-ras基因的表达情况,并分析其意义。结果PITX1在肝癌细胞(HepG2、SMMC-7721)中表达比正常肝细胞L02显著降低,Pan-ras在肝癌细胞(HepG2、SMMC-7721)中的表达与正常肝细胞L02相比显著升高。结论PITX1在肝癌细胞中的低表达,以及Pan-ras的高表达,可能导致肝癌无限增殖,构成了肝癌细胞信号传导网络中的重要一环。 Objectives To investgate the expression of PITX1 and Pan-ras in L02, HepG2 and SMMC-7721 cell lines. Methods PITX1 and Pan-ras protein expression was detected using immunohistochemistry and western blot and semiquantitative RT-PCR respectively in L02,HepG2 and SMMC-7721 cell lines. Results Compared with L02 cell, PITX1 were significantly decreased in HepG2 and SMMC-7721 cell lines（P 〈0.05） , Pan-ras expression in HepG2 and SMMC-7721 cells was significantly higher than in L02 cells（P 〈 0.05）. Conclusions The expression of PITX1 and Pan-ras maybe induce cell to propagate infinitely in HCC.
作者 廖家智 丁静丽 田德安 王波 王南下 但自力 杨玉珍 刘梅 王家駹
机构地区 华中科技大学同济医学院附属同济医院消化内科
出处 《胃肠病学和肝病学杂志》 CAS 2007年第4期338-340,共3页 Chinese Journal of Gastroenterology and Hepatology
关键词 肝细胞癌 PITX1基因 Pan-ras基因 Hepatocellular carcinoma PITX1 Pan-ras
分类号 R735.2 [医药卫生—肿瘤]
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参考文献8

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共引文献20

同被引文献12

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胃肠病学和肝病学杂志
2007年 第4期

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