哮喘患者单个核细胞Th1／Th2和IFN-γ及IL-4 mRNA表达

Detection of Th1/Th2 and IFN-γ, IL-4 mRNA Expression in the Peripheral Blood Mononuclear cell of the Patient with Asthma

目的探讨外周血单个核细胞Th1／Th2在哮喘发病中的作用和IFN-γ及IL-4 mRNA表达的情况。方法分别用流式细胞仪及RT-PCR方法检测97例哮喘患者及20例健康人外周血单个核细胞（PBMCs）Th1／Th2水平和IFN-γ及IL-4 mRNA表达。结果哮喘组外周血Th1细胞百分比低于对照组（P〈0．01），而Th2百分比高于对照组（P〈0.01）；哮喘组PBMCS IFN-γ的mRNA表达水平与对照组差异无统计学意义（P〉0．05），而IL-4 mRNA的表达显著高于对照组（P〈0．01）。结论哮喘患者体内存在Th1／Th2的失衡，并提示哮喘属于Th2优势疾病。

Objective To investigate the possible roles of Th1/Th2 in patients of asthma. Methods The condition of Th1/Th2 was detected by flow cytometry, the IFN-γ and IL-4 mRNA expression in PBMCs were examined by RT-PCR in 97 patients with asthma and 20 healthy pepole. Results The propotion of Th1 in patients was lower （P〈 0.01） and the propotion of Th2 in patients was higher than those in the control group （P〈0.01）, IL-4 mRNA expression in PBMCs of the patients with asthma was significantly higher than that of the control group （P〈 0.01）, but the difference of the IFN-γ mRNA expression was not statistical between two droups（P〉0.05）. Conclusion There was Th1/Th2 imbalance in the patients with asthma, and the overexpression of Th2 may play an important role in the mechanism of asthma.