摘要
目的:探讨维生素B12片剂中维生素B12的含量测定方法。方法:紫外可见分光光度法。样品以标准曲线法为测定含量依据,在361nm的波长处测定吸光度。结果:维生素B12在5μg/mL～100μg/mL浓度范围线性关系良好。回归方程Y=0.0193X+0.048,r=0.9937。结论:测定的5个不同批号样品其含量均在标示范围之内,该方法简便、准确、灵敏度高。
Objective：The measurement method of V- B12 content in tablet is established. Methods：UV- Vis The absorption is measured at 361 nm according to calibration carve method. Results ：The linear range is in 5 μg/mL - 100 μg/mL and the regression equation is Y = 0. 0193X ＋ 0. 048, r = 0. 9937. Conclusion ： The mea- surement is done in 5 kind of lot number and the result indicate that the V - B12 content in tablet corresponds with standard range. This method is simple, accurate and high sensitivity.
出处
《长治医学院学报》
2007年第4期251-253,共3页
Journal of Changzhi Medical College
