两种不同滤材去白细胞滤器对全血过滤的影响

The Effect of Two Leukocyte Depletion in-line Filters on the Efficiency of Whole Blood Filtration

我们研究了由两种不同方法表面处理的滤材制备的去白细胞滤器(A和B)对全血过滤性能以及对血浆蛋白和部分凝血因子的影响。取当天采集的ACD-B抗凝全血(n=10),置4℃冷藏后,于采集后6h内过滤。取过滤前、后的血样计数血细胞、分离血浆,测定血浆蛋白和部分凝血因子活性。10单位400mlACD-B抗凝全血过滤后剩余白细胞数均小于2.5×106,红细胞回收率大于93%。滤器A的过滤速度比滤器B快(8'01"-A,9'29"-B);对过滤前、后血浆总蛋白、白蛋白、IgG、IgM、纤维蛋白原、VIII因子、IX因子、vWF和补体C3测定(n=8)表明两种滤器过滤前、后均无显著差异(P>0.05)。而对V因子、XI因子和AT-III测定发现,经以上两种滤器过滤后活性有显著下降(P<0.05)。滤器B与滤器A相比IgA和V因子的活性降低更多。这可能与两者不同的材料表面特性有关。两种材料制成的滤器均能有效滤除全血中的白细胞,过滤后全血和从中分离的新鲜冰冻血浆仍符合血液质量要求。然而,在过滤全血时为保持血浆蛋白的含量和凝血因子的活性,应选择血液相容性较好的滤材。

The aim of this study was to observe the difference in respect to the leukocyte reduction efficiency and quality of fresh-frozen plasma （FFP） from filtered whole blood between two types of in-line filters wherein only filter materials were surface modified by the two methods respectively. Whole blood was kept in refrigerator and filtered within 6 h of collection at ambient temperature. Samples were taken pre- and post filtration for analysis of WBC numbers, coagulation factors and complement activation （n = 8 for each type of filter）. All filtered units contained 〈2.5× 10^6 residual leucocytes. RBCs recovery was over 93%. No significant difference between group A and B was seen. But group B appeared to take longer time for filtration than did group A（9′29″ vs. 8′01″）. Neither group A nor group B showed statistically significant losses of total protein, album, IgG, IgM, fibrin, factors Ⅷ, Ⅸ, vWF and C3（P〉0.05）. Factor Ⅴ, Ⅺ and AT-Ⅲ decreased significantly m two group filters. Group B showed more significant losses of IgA content and factor Ⅴ activity than did group A, which appeared to be related to the difference in surface character between group A and group B filters. These two types of filters could remove leukocytes effectively, and no significant changes were observed in the quality of FFP from the filtered whole blood. It is presumed that the filter material with better bio-compatibility will give a high recovery of plasma protein and coagulation factors after filtration.