实验性家兔骨折愈合过程中破骨细胞的形态学测量

Morphometry of Osteoclasts in Experimental Fracture Healing of Rabbits

为了探讨中成药生骨再造散对骨折愈合中骨吸收与破骨细胞形态学特征的影响及二者的关系,将30只青紫蓝家兔建成双侧桡骨3mm骨缺损的骨折模型,随机分为生骨再造散组(A组)、仙灵骨葆组(B组)及对照组(C组),每组各10只,于术后14d和31d每组各处死动物5只取材,进行骨吸收与破骨细胞形态学计量检测和相关性分析。结果:术后A组骨吸收较对照组强,破骨细胞形态学也发生了一定变化,差异有统计学意义(P<0.05);B组对破骨细胞形态也有一定影响,但对骨吸收影响不大。相关分析表明:骨吸收主要与破骨细胞数目相关,其次还与破骨细胞形状因子、面积、光密度等相关。实验结果提示,生骨再造散在术后14d和31d均可促进骨吸收,可能是通过早期提高破骨细胞数量,后期则增强其功能实现的,对骨折愈合有一定促进作用。

This study was designed to investigate the effects of some Traditional Chinese Medicine （TCM） agents on bone resorption and morphometric features of osteoclasts as well as their relationships. TCM ShengGuZaiZaoSan and XianLingGuBao, were used to treat the experimental fracture. Thirty 6-month-old Chinchilla rabbits were used for the establishment of animal models each with a 3 mm bone defect in the middle of left radius as well as of right radius. These models were divided randomly into 3 groups ： ShengGuZaiZaoSan Group （Group A）,XianLingGuBao groups （Group B） and control-group （Group C）. Every group was further divided into 2 subgroups： a former sacrificed group （14 days after operation） and a latter sacrificed group （31 days after operation ）. After the rabbits being killed, the samples of their undecalcified calli were subjected to the morphometry study of bone resorption and osteoclasts. Group A had more bone resorption,compared with Group B and C. Both Groups A and B exhibited some changed morphometric features of osteoclasts as compared with Group C（P〈0. 05）. Simple correlation analysis indicated that bone resorption is mainly correlated with osteoclast numbers,and that in individual group, bone resorption is correlated with osteoclast form factor,area and mean photodensity （P〈0. 05）. These allow us to conclude that ShengGuZaiZaoSan can increase bone resorption and accelerate bone remodeling by increasing osteoclast numbers at the former stage and can enhance osteoclast function at the latter stage. These changes are beneficial to fracture healing.