摘要
目的建立测定小鼠血液乙醇的浓度的灵敏方法。方法取仲丁醇标液1ml和血样0.25ml放入顶空瓶中,用内衬聚四氟乙烯膜的硅垫密封,置电热恒温器中60℃加热平衡30min,气相色谱仪测定小鼠血液的乙醇浓度。结果校正因子Fi/Fs的平均值为3.686,平均偏差为2.15%。结论本方法是测定血液乙醇浓度简便、灵敏、有效的方法。
Objective： To detect the alcohol concentration in Rats＇ blood. Methods： Sec -butyl alcohol is used as the internal standard and, heated at the temperature of 60℃ and balanced for 30 minutes. Headspace gas chromatograph is adopted to detect the alcohol concentration in Rats＇blood. Results： The average value of correction factor Fi/Fs is 3. 686, and the average deviation is 2. 15%. Conclusions： Headspace gas chromatograph is the simple, flexible and effective method to detect the alcohol concentration in Rats blood.
出处
《湖北中医学院学报》
2007年第2期37-38,共2页
Journal of Hubei College of Traditional Chinese Medicine
关键词
顶空气相色谱法
血药浓度
乙醇
仲丁醇
Headspace gas chromatograph
Blood concentration
Alcohol
Sec - butyl alcohol
