摘要
研究了桔青霉生产核酸酶P1的适宜培养基和培养条件.培养基组成为:葡萄糖7%,蛋白胨1%,玉米浆0.2%,磷酸氢二钾0.05%,磷酸二氢钾0.05%,氯化钙0.04%,硫酸锌0.02%,硫酸镁0.03%,硫酸锰0.05%,pH5.4,30℃发酵66h酶活达最高值,粗酶液酶活为400IU/mL.
The suitable conditions have been established for nulease P 1 produced by P. citrinum. Composition of the medium is as following: glucose 7%, petone 1%, corn milk 0.2%, K 2HPO 4 0.05%, KH 2PO 4 0.05%, CaCl 2 0.04%, ZnSO 4 0.02%, MgSO 4 0.03%, MnSO 4 0.05%, pH is 5.4. Under these conditions the activity of P. citrinum cultures incubated on rotary shaker at 30℃ for 66 hours are as high as 400 IU/mL.
出处
《烟台大学学报(自然科学与工程版)》
CAS
1997年第2期106-109,共4页
Journal of Yantai University(Natural Science and Engineering Edition)