漆树品种的AFLP分析及评价(简报)

GENETIC EVALUATION OF TOXICODENDRON VERNICIFLUUM CULTIVARS USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS

漆树（Toxicodendron vernicifluum（stokes）F．A．Barkley）隶属于漆树科（Anacardiaceae）漆树属（Toxicodendron）的落叶乔木，是我国重要的特用经济林木。漆树栽培与生漆使用在我国已有几千年的历史，在长期栽培过程中形成了许多农家品种，它们具有一定的形态特点，适应一定的生长环境，并具有产漆量高、生漆品质好等特性。

Accurate identification of Toxicodendron vernicifluum varieties and understanding their genetic relationships are essential for the improved varieties selection. In this study, Amplified fragment length polymorphism （AFLP） markers was used to evaluate the genetic resources of nine Toxicodendron vernicifluum cuhivars from shaanxi. Eight pairs of AFLP primers with Mse Ⅰ fluorescent labeled were screened from EcoR Ⅰ/Mse Ⅰ primer combinations. Different pair of primers differs widely to detect genetic polymorphism of Toxicodendron vernicifluum. The polymorphic bands detected by each pair of primers ranged from 242 to 622 with average value of 350.88. Eight pairs of AFLP primers produced 4463 bands in total. Among them, 2807 bands （62.64%） were polymorphic. genetic similarity coefficient （SC） among all cultivars varied from 0.673 to 0.833, which further verified that only a little genetic diversity existed among the cultivars. It was the first time that AFLP technique was applied to identify Toxicodendron vernicifluum cultivars. The study suggested that AFLP technique is a useful tool for individual identification of Toxic ode ndron vernicifluum cultivars and could be applied to the Toxicodendron vernicifluum＇ fingerprinting work in China.