摘要
本文报道4例副肿瘤性小脑变性患者血清中存在特异性抗小脑浦肯野细胞抗体,免疫组化示小脑浦肯野细胞呈特征性粗颗粒状染色,患者血清中抗体滴度为1∶8000~1∶32000。免疫印迹技术发现抗体识别小脑浦肯野细胞提取物中分子量为34000与62000两种蛋白抗原,认为这种抗体是特异性抗-Yo抗体。本文就抗-Yo在临床检查中的意义进行了讨论。
To discuss the significance of clinical test of Anti-Purkinje cell antibody.Methods the immunological character of Anti-Purkinje cell antibody in the sera from 4 patients with paraneoplastic cerebellar degeneration (PCD) was observed by the indirect immunoperoxidase methods and Western blot analysis. Results The sera from 4 patients with PCD reacted with the frozen sections of human cerebella and produced coarse cytoplastic granular staining of Purkinje cells by the indirect immunoperoxidase methods, with the titre of 1: 8 000 to 1: 32 000. By Western blot analysis the sera from all patients recognized two of 34, 62 kd antigens extracted from cerebellar Purkinje cells. Conclusion The anti-purkinje cell antibodies from these patients were anti-Yo antibodies.
出处
《中国神经免疫学和神经病学杂志》
CAS
1997年第2期96-99,共4页
Chinese Journal of Neuroimmunology and Neurology
基金
国家教委回国人员科研基金
关键词
免疫印迹技术
神经系肿瘤
副肿瘤性
小脑变性
immunohistochemistry
Western blot analysis
anti-Purkinje cell antibody
paraneoplastic cerebellar degeneration