巴曲酶促进小鼠腹腔巨噬细胞吞噬过氧化低密度脂蛋白

Accelerating effects of batroxobin on the phagocytosis of pox LDL by mouse peritoneal macrophages

采用小鼠腹腔巨噬细胞在含有过氧化低密度脂蛋白（Ｐｏｘ－ＬＤＬ）的培养液中培养，并用巴曲酶（ｂａｔｒｏｘｏｂｉｎ）进行干预，运用透射电子显微镜进行观察，旨在了解巴曲酶对巨噬细胞吞噬ｐｏｘ－ＬＤＬ的影响。研究结果表明；小鼠腹腔巨噬细胞与纯培养剂ＤＭＥＭ正常低密度脂蛋白（ｎ－ＬＤＬ），以及ｎ－ＬＤＬ加巴曲酶培养４小时后，细胞的超微结构与培养前相似，而与Ｐｏｘ－ＬＤＬ一起培养４小时后细胞胞浆内则含大量的脂滴，加入巴曲酶及小鼠腹腔巨噬细胞内脂滴的生成明显加快。

Mouse peritoneal macrophages were incubated in DMEM medium with batroxobin (DF 521) to determine the effect of batroxobin on the internalization of peroxidized low density lipoprotein (pox LDL) by transmission electron microscopy. Although the morphology of the mouse peritoneal macrophages after incubation with DMEM, normal LDL(n LDL) and n LDL plus batroxobin was similiar to that of the cells before incubation, they exhibited numerous cytoplasmic lipid droplets after incubation with pox LDL for 4 hours. After addition of batroxobin to the medium containing pox LDL, the production of lipid droplets in the mouse peritoneal macrophages was tremendously acccelerated. Conclusion:batroxobin accelerates the phagocytosis and degradation of pox LDL by macropages.