CCK-8检测法研究槲皮素对人Tenon囊成纤维细胞的抑制作用

Inhibitory effect of quercetin on human Tenon’s capsule fibroblasts

目的观察槲皮素(quercetin,QU)对体外培养的(human Tenon’s capsule fibro-blasts,HTCF)增殖的抑制作用。方法体外培养HTCF。配制QU溶液,其浓度依次为20μmol·L-1、40μmol·L-1、60μmol.L-1、80μmol·L-1、100μmol·L-1,采用活细胞计数试剂盒CCK-8检测不同浓度QU作用下HTCF的增殖,并在光学显微镜下观察细胞的形态变化。结果不同药物浓度HTCF形态学检测显示:50μmol·L-1QU作用48h后,细胞收缩,胞核固缩,聚集在核膜边缘;100μmol·L-1QU作用48h后,细胞未见增殖,出现凋亡小体,细胞的增殖几乎完全被抑制。细胞生长抑制实验的结果显示:QU在各浓度组对体外培养的HTCF均有抑制作用,抑制效应呈剂量时间依赖关系。结论QU能抑制体外培养的HTCF的增殖,且该作用呈剂量和时间依赖性。

Objective To investigate the inhibitory effect of quercetin（QU） on proliferation of human Tenon＇ s capsule fibroblasts. Methods Human Tenon＇ s capsule flbroblasts were cultured in vitro and exposed to different concentrations of QU solution,which were 20 μmol · L^-1 ,40 μmol · L^-1 ,60 μmol · L^-1, 80 μmol · L^-1and 100 μmol · L^-1. The proliferation of human Tenon＇ s capsule flbroblasts was detected by CCK-8 agent,and the shape changes of cell were observed with light microscope. Results After Cells were treated by 50 μmol · L^-1 QU for 48 hours, we observed that cells were shrunk and aggreagted to nuclear membrane edge. After cells were treated with 100 μmol/L QU for 48 hours, the proliferation of human Tenon＇ s capsule flbroblasts was completely inhibited,and apoptotic bodies were seen. We could observe apoptotic body increased and the proliferation of human Tenon＇ s capsule flbroblasts was completely inhibited. Conclusion QU can inhibit the proliferation of human Tenon＇ s capsule flbroblasts in vitro and show a dose-time dependent tendency. It is potentially usefitl for preventing scar formation in the glaucoma filtration surgery.