氨基酮戊酸光动力作用诱导人宫颈癌Hela细胞凋亡的实验研究

Study on apoptosis of human cervical cancer Hela cell induced by aminolevulinicacid-mediated photodynamic therapy

目的:探讨光动力疗法(PDT)对体外培养的人宫颈癌细胞系Hela的杀伤效应。方法:将经不同浓度5-氨基酮戊酸(ALA)处理的Hela细胞照射不同剂量的激光,用MTT法测定OD492值,细胞染色观察形态学改变,流式细胞术检测细胞周期及凋亡率。结果:ALA10-1mM和激光剂量20J/cm2是PDT对Hela细胞杀伤作用的最佳参数。流式细胞术显示PDT组发生了G0-G1期生长停顿,凋亡率为(22.6±4.56)%,显著高于对照组。结论:ALA-PDT通过诱导凋亡对体外培养的Hela细胞产生杀伤作用。

Objective：To observe the effects of photodynamic therapy（PDT） on human cervical cancer cell line Hela in vitro.Methods：The cultured cells were treated with different concentrations of aminolevulinicacid（ALA） and different dosages of laser,then MTr colorometric assay was applied to measure the OD492.Cellular morphology was observe by May-Grtinwald-Farbstoff Giemsa staining.Cell cycle and apoptosis rate were estimated by flow cytometry.Results：The parameters of PDT is optimal when ALA was 10^-1mM and laser dosage was 20 J/cm^2.Flow cytometry showed that PDT group had GO/G1 stage arrest.The apoptosis rate in PDT group was （22.6＋4.56）%.h was higher than that in control group.Conclusion：ALA-PDT has a significant killing effect on Hela cells by induction of apoptosis.