不同辣椒材料离体再生及其影响因素的研究

In vitro Regeneration and Its Influential Factors in Pepper (Capsicum annuum L.)

以8个辣椒（Capsicum annuum L.）纯系为材料,对不同材料、外植体种类和激素组合等因素对辣椒植株离体再生的影响进行了研究.结果表明,较高的6-BA/IAA值有利于辣椒外植体的分化再生,而6-BA/IAA值较低则适合于再生芽的伸长;不同辣椒材料的再生能力差别较大;辣椒带柄子叶再生能力比下胚轴强,是较好的外植体材料;12～16 d苗龄的外植体分化频率较高;在供试的8个辣椒材料中‘2096’、‘B4’和‘B7’的再生能力较强.高频率的不定芽分化培养基为MB（MS无机成分＋B5有机成分）＋0.8 mg/L IAA＋5.0 mg/L 6-BA＋4.0 mg/L Ag-NO3;不定芽伸长的培养基为MB＋0.8 mg/L IAA＋2.0 mg/L 6-BA＋2.0 mg/L GA3＋4.0 mg/L AgNO3;高效生根诱导培养基为MB＋0.2 mg/L IAA＋0.1 mg/L NAA.

In vitro plant regeneration was achieved from eight pepper materials （Capsicum annuum L. ）. The effects of genotype, various explant types （cotyledons, hypocotyls） and hormones were evaluated. It was shown that the high 6-BA/IAA ratio was conducive to the differentiation and the low 6-BA/IAA ratio was suitable for the elongation of the regenerative buds ;different pepper materials differed greatly in their regeneration capacities. The cotyledons had stronger regenerative capacity than that of the hypocotyls, and the explants from 12- 16 day old seedlings had a high differentiation rate. Higher regeneration frequency was obtained from materials of ‘2096＇,‘ B4＇ and ‘B7＇. The optimal medium for the differentiation of pepper buds was Murashige and Skoog （MS）medium supplemented with B5 organic nutrient（MB）,0.8 mg/L IAA,5.0 mg/L 6-BA and 4. 0 mg/L AgNO3. The best media for shoots elongation was MB medium with 0.8 mg/L IAA,2.0 mg/L 6-BA,2.0 mg/L GA3 and 4.0 mg/L AgNO3 and the rooting medium was MB medium with 0.2 mg/L IAA,0.1 mg/L NAA.