湿地松体细胞胚胎发生和植株再生

Plantlet regeneration via somatic embryogenesis in slash pine

以湿地松（ＰｉｎｕｓｅｌｉｏｔｉＥｎｇｅｌｍ．）的未成熟合子胚为外植体，在附加８ｍｇ／Ｌ２，４Ｄ和４ｍｇ／ＬＢＡ的ＬＰ培养基上诱导出胚性愈伤组织。在含１ｍｇ／Ｌ２，４Ｄ和０５ｍｇ／ＬＢＡ的培养基上保持并增殖。提高培养基的渗透压后，愈伤组织内形成大量的胚性胚柄细胞团和早期原胚。在附加４ｍｇ／ＬＡＢＡ，７５ｇ／ＬＰＥＧ和５ｇ／Ｌ活性炭的ＬＰ培养基上，早期原胚发育形成粗壮的子叶胚。在无激素ＬＰ培养基上，成熟的体细胞胚萌发并发育形成再生完整植株。体细胞胚转换成小植株的频率为１５６％。

Embryogenic callus was initiated from immature zygotic embryos of slash pine ( Pinus elliottii Engelm.) cultured on LP medium containing 8 mg/L 2,4 D and 4 mg/L BA. Multiplication can be achieved on LP medium with 1 mg/L 2,4 D and 0.5 mg/L BA. Embryonal suspensor masses and early stage proembryos were formed from embryogenic callus cultured on high osmotic pressure medium. Early stage embryos developed into cotyledonary embryos on LP medium supplemented with 4 mg/L ABA, 75 g/L PEG and 5 g/L activated charcoal. Mature somatic embryos germinated into regeneration plantlet on medium with hormone free. The frequency of somatic embryos converted into regeneration plantlets is 15.6%.