盖诺与顺铂联合化疗加同期放射对乳腺癌MCF-7细胞作用的研究

Study of NVB and DDP chemotherapy with concurrent radiotherapy on breast cancer MCF-7 cells

目的:探讨盖诺(NVB)联合顺铂(DDP)的放化同期治疗乳腺癌的有效性、时机选择和作用机制。方法:NVB和DDP(NP方案)对乳腺癌MCF-7细胞联合化疗同期放射。比较照射加联合化疗组和单纯照射组、不同间隔时间联合化放疗的细胞存活率(SF)、凋亡和细胞周期阻滞差异。结果:联合化疗加同期放射后SF较单独放疗更低。化疗处理后间隔4、12和36h照射的SF值最低,0h居中,而间隔48和72h后,SF值明显上升,放化疗协同杀灭效应减弱,SF最低与最高值相差约15倍。联合化疗加照射组细胞凋亡指数(AI)明显高于单独放射和联合化疗组。6、8Gy放射后36h时,放化疗联合组与单独放射组比较AI分别高达30.7%、38.35%和2.22%、3.27%,差异有统计学意义,P<0.05。化疗处理MCF-7细胞1h后在间隔4、12、36和72h处,G2/M周期细胞比例较高,0、48h处较低。结论:联合化放疗对乳腺癌MCF-7细胞具有较强的协同杀灭作用,化放联合治疗的时机选择在肿瘤细胞杀灭过程中具有重要的意义,这种协同杀灭作用与增加细胞凋亡率和化疗造成的细胞G2/M周期阻滞有关。

OBJECTIVE： To explore the effectivity of concur-rent radiotherapy and chemotherapy, opportunity chosing and mecha-nism on breast cancer MCF-7 cells. METHODS： The results of sur- vival fractions （SF） among the groups of combination of NVB and DDP with concurrent radiotherapy, chemotherapy and radiotherapy alone were compared. Further researches on apoptosis, cell cycle ac-cumulation within these groups were done. RESULTS： SF of the group combined chemotherapy with radiation was much lower than that of group of radiotherapy alone. The SFs of MCF-7 cells irradia- ted at 4 h, 12 h and 36 h after the chemotherapy were the lowest. SF at 0 h was middling. SFs at 48 h and 72 h after the chemotherapy were found rising greatly. The highest SF was about 15 times of the lowest. Apoptosis index （AI） of the combination group of chemo-therapy and radiation was much higher than those of groups of chem- otherapy and radiation alone. Especially at 36 h after 6 Gy or 8 Gy by being irradiated, AIs of the combination group of chemotherapy and radiation were 30.7% and 38. 350/60 while AIs of group of radiation a-lone were 2.22% and 3.27%, P〈0.05. G2/Ms of MCF-7 cell at 4, 12, 36 and 72 hours after chemotherapy were higher compared with those at 0 and 48 hours later. CONCLUSIONS： Teh combination of concurrent chemotherapy with radiotherapy can make much stronger cooperated killing action on MCF-7 cells, Opportunity of the combi-nation treatment takes a great significance during this process, This kind of cooperated killing is related to the increase of apoptosis from combination of chemotherapy with radiation and cell cycle accumula-tion at G2/M caused by chemotherapy.