超临界CO_2萃取香椿叶总黄酮

Extraction of Flavonoids from Toona sinensis (A Juss.) Roem Leaves by Supercritical CO_2 Fluid

正交实验法优化了超临界CO2萃取香椿叶总黄酮的工艺。在原料50 g,分离室Ⅰ温度35℃、压力7 MPa;分离室Ⅱ温度35℃、压力与储罐平衡条件下,超临界CO2萃取香椿叶中总黄酮的最佳工艺条件为:萃取压力30MPa,萃取温度45℃,萃取时间2.5 h,夹带剂用量为3 mL/g原料,CO2流量35 L/h,提取5次,前2次用无水乙醇、后3次用体积分数为85%的乙醇做夹带剂。在此条件下1 g香椿叶中提取的总黄酮质量为8.8369 mg,总萃取率为51.06%,提取物以总黄酮计的清除2,2-二苯代苦味酰基苯肼基(DPPH)自由基的IC50为5.080 g.g-1。

The extraction process for total flavonoid from Toona sinensis （A Juss. ） Roem leaves by supercritical CO2 fluid was optimized： extraction pressure 30 MPa, extraction temperature 45 ℃, extraction time 2.5 h, entrainer dosage 3 mL/g, supercritical CO2 flux 35 L/h, extracting for five times, former two times with anhydrous ethanol and latter three times with 85 % ethanol as entrainer. Yield of total flavonoid from Toona sinensis （A Juss. ） Roem leaves was 8. 836 9 mg/g, the extraction rate was 51.06%. IC50 of scavenging DPPH free radicals by total flavonoid was 5. 080 g DPPH/g flavonoid.