基于基因芯片技术探讨脑缺血病理分子基础及其药物作用机制的研究进展

Advances in the study of gene chip technology for the investigation of the mechanisms underlying cerebral ischemia and anti-cerebral ischemia agents

基因组学是随分子生物学发展应运而生的一门重要学科,是从整体水平对基因组高通量、高集成、高平行性、微型化和自动化的综合分析。脑卒中是危害人类健康的重要脑血管疾病之一。应用基因芯片技术对脑卒中进行研究,不仅可在基因水平上揭示疾病的本质,还有助于全面探讨脑卒中的病理分子机制,发现药物治疗靶点,开发治疗新药。本文以基因芯片技术运用于实验性脑缺血的体内研究成果为重点,并结合本实验室的工作探讨脑缺血病理分子基础及其药物作用机制的研究进展。

With the development of molecular biology,genome science becomes an important subject currently.Characterized by high-throughput,high-integration,high-parallelism,miniaturization and automation,it is the integrated study of gene properties on a large scale.Stroke,an important cerebral vascular disease,is one of the threats to human health.The utilization of microarray study for the pathogenesis of stroke,not only reveals the essentials of the disease in the overall level of genes,but also contributes to the detection of therapeutic targets and the development of novel drugs for stroke.Referring to our own work,this discussion focuses on the progress of the mechanisms underlying experimental cerebral ischemia investigation in vivo as well as anti-cerebral ischemia agents by gene chip technology.