摘要
从5种变质豆浆中分离得到3株腐败菌株S1、S2和S3。3株菌均能在1×10^5Pa、30min杀菌条件下和添加300mg/kgNisin杀菌条件下存活。对分离菌株进行了菌落形态、生理生化特征和16SrDNA序列分析,鉴定结果表明3株菌均为革兰氏阴性细菌,分别为地衣芽孢杆菌(Bacillus licheniformis)、短小芽孢杆菌(Bacillus pumilus)和短芽孢杆菌(Brevibacillus borstelensis),GenBank登录号为EF439666-EF439668。
In this paper, three spoilage organisms were separated from five transmutative soy milks, and all the three spoilage boetena could survive condition of both 1 × 10^5Pa, 30min and 300mg/kg Nisin. Morpha character, physiological and biochemical characteristics, and a phylogenetic analysis based on 16S rDNA gene sequences reveal that these three strains are Bacillus licheniformis, Bacillus pumilus and BrevibaciUus borstelensis respectively. GenBank accessions for these three strains are EF439666-EF439668.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第4期621-624,共4页
Microbiology China
基金
上海市教育基金会及上海科技成果转化促进会资助项目
上海市重点学科建设资助项目(No.T1102)
上海市教委青年基金项目(No.04KC13)。
关键词
豆浆
腐败微生物
分离
鉴定
生理生化特征
16S
RDNA
Soy milk, Spoilage organisms, Isolation, Identification, Physiological and Biochemical Characteristics, 16S rDNA