摘要
从纺织厂的车间污水分离获得菌株WSHDZ-01,其产过氧化氢酶酶活为600U/mL,酶活的pH范围为5.0-12.0。根据对该过氧化氢酶生产菌的形态和生理生化特征的分析和16SrDNA序列分析,鉴定该菌为枯草芽孢杆菌。通过对该菌株发酵培养基中碳氮源的优化,其最佳碳源为10g/L的葡萄糖,氮源为5g/L的NaNO3,在此条件下产酶达3258U/mL。此外,该菌株所产过氧化氢酶的最适反应pH为11.0,最适温度为55℃,在pH11.0和50℃下保持15min后,剩余酶活仍达98%以上。
A strain named as WSHDZ-01 with high capability of producing alkaline catalase(CAT) was isolated from the wastewater discharged from a textile factory. The catalase from this strain was active in the range of pH 5.0-pHI2,0, and the highest enzyme activity was observed as 600 U/mL at pH 7.0. According to the physiological and biochemical characteristics and 16S rDNA sequence of WSHDZ-01, the strain was identified as Bacillus subtilis. The optimal carbon source and nitrogen source were also determined as 10 g/L glucose and 5 g/L NaNO3, respectively. The highest enzyme activity of 3258 U/mL was achieved after the optimization. The resultant catalase reacted best at 55 ℃ and pH 11.0, and had activities 〉 98% after 15 min maintenance under 50℃ and pH 11.0.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第4期667-671,共5页
Microbiology China
基金
国家高技术研究发展计划("863"项目)资助(No.2003AA322050)
关键词
枯草杆菌
筛选
鉴定
发酵优化
酶学性质
Bacillius, Screening, Identification, Fermentation optimization, Enzymatic properties