摘要
基于双抗体夹心ELISA反应原理,在两种不同加工成形的硝酸纤维素膜条(NC strip)上进行了马铃薯A病毒(PVA)的检测研究,并以酶标板ELISA做参比。结果表明,在NC条-2(NC strip-2)上的检测灵敏度与酶标板ELISA相当,而反应试剂的用量仅为酶标板ELISA的百分之一;NC条-1(NC strip-1)由于加样点间易发生交叉污染而不适合进行ELISA检测。应用NC条-2可稳定进行PVA的ELISA检测,为进一步开展微流体斑点免疫检测研究奠定了基础。
Based on DAS-ELISA procedures, Dot-ELISA for detecting potato virus A(PVA) was conducted on two kinds of differently processed NC (nitrocellulose) strips (NC strip-1, NC strip-2), respectively. The PVA in infected potato leaves can be clearly detected by performing the ELISA on NC strip-2, whereas not on NC strip-1 due to cross-reaction between two adjacent NC dots. This result has given a deep foundation for developing micro fluidic immunoassay chip for potato virus A.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第4期712-714,共3页
Microbiology China
基金
科研院所社会公益研究专项(No.2005DIA2T128)
国家质检总局科研计划项目(No.2005IK0073)